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Michael V. Graves

Michael Graves
Michael V. GravesAssociate Professor & Associate Chair

Research Interests

Molecular Biology and Virology

Research in my lab focuses on the chlorella viruses that infect chlorella-like algae. They are distributed worldwide and many genetically distinct isolates populate a single water sample. Their genome is unusually large. One isolate, PBCV-1, for example, (genome size = 330744 bp dsDNA) codes for ~375 proteins, nearly half of which resemble proteins of known function. The genomes of other chlorella virus isolates are probably co-linear with PBCV-1 and appear to share many, but not all, genes. This suggests that the entire "gene pool" of this genus exceeds the 375 PBCV-1-encoded genes. Many of these genes encode proteins (e.g., enzymes involved in protein glycosylation) rarely associated with viruses.

Education

  • Ph D: Molecular Genetics, Virology, (1992), Oregon State University - Oregon
    Dissertation/Thesis Title: Analysis of two highly expressed genes from Chlorella virus PBCV-1: Protein characterization and the DNA sequences of the major capsid protein gene and the early/late 33-kDa protein gene.
  • BS: Biology, (1986), University of Nebraska, Lincoln. - Lincoln, Nebraska

Awards and Honors

  • Teaching Appreciation Award (2008) - Student Government Association, University of Massachusetts, Lowell
  • Teaching Excellence Award (2005) - Department of Biological Sciences, University of Massachusetts, Lowell.
  • Phi Beta Kappa (1986) - University of Nebraska, Lincoln, Alpha Chapter

Publications

  • Schneider, W.L., Damsteegt, V.D., Stone, A.L., Kuhlmann, M., Bunyard, B.A., Sherman, D.J., Graves, M.V., Smythers, G., Smith, O.P., Hatziloukas, E. (2011) "Molecular analysis of soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination," Virology 412:1 pp. 46-54
  • Van Etten, J.L., Gurnon, J.R., Yanai-Balser, G.M., Dunigan, D.D., Graves, M.V. (2010) "Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins independent of the endoplasmic reticulum and Golgi," Biochimica et Biophysica Acta - General Subjects 1800:2 pp. 152-159
  • Nguyen, P.s., Falcone, D.L., Graves, M.V. (2010) "The A312L 5'-UTR of Chlorella virus PBCV-1 is a translational enhancer in Arabidopsis thaliana," Virus research 140:1-2 pp. 138-146
  • Van Etten, J.L., Graves, M.V. (2008) "Algal Viruses (Phycodnaviridae)," Virology 1:
  • Fitzgerald, L.A., Boucher, P.T., Yanai-Balser, G.M., Suhre, K., Graves, M.V., Van Etten, J.L. (2008) "Putative gene promoter sequences in the chlorella viruses," Virology 380:2 pp. 388-393
  • Tchantchou, F., Graves, M.V., Falcone, D., Shea, T.B. (2008) "S-adenosylmethionine mediates glutathione efficacy by increasing glutathione S-transferase activity: Implications for S-adenosyl methionine as a neuroprotective dietary supplement," Journal of Alzheimers Disease 14:3
  • Shaughnessy, P., Livingston, G., Graves, M.V. (2008) "Towards predicting protein-protein interactions in novel organisms.," International journal of computational biology and drug design 1:3 pp. 235-253
  • Fitzgerald, L.A., Graves, M.V., Li, X., Hartigan, J., Pfitzner, A.J., Hoffart, E., Van Etten, J.L. (2007) "Corrigendum to "Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfacea" [Virology 362 (2007) 350-361] (DOI:10.1016/j.virol.2006.12.028)," Virology 366:1 pp. 226
  • Fitzgerald, L.A., Graves, M.V., Li, X., Hartigan, J., Pfitzner, A.J., Hoffart, E., Van Etten, J.L. (2007) "Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfacea," Virology 362:2 pp. 350-361
  • Fitzgerald, L.A., Graves, M.V., Li, X., Feldblyum, T., Hartigan, J., Van Etten, J.L. (2007) "Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi.," Virology 358: pp. 459-471
  • Fitzgerald, L.A., Graves, M.V., Li, X., Feldblyum, T., Nierman, W.C., Van Etten, J.L. (2007) "Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A," Virology 358: pp. 472-484
  • Tchantchou, F., Graves, M.V., Shea, T.B. (2006) "Expression and activity of methionine cycle genes are altered following folate and vitamin E deficiency under oxidative challenge: Modulation by apolipoprotein E-deficiency," Nutritional Neuroscience 9:1-2 pp. 17-24
  • Tchantchou, F., Graves, M.V., Ortiz, D., Chan, A., Rogers, E., Shea, T.B. (2006) "S-Adensosyl Methionine: A Connection Between Nutritional and Genetic Risk Factors for Neurodegeneration in Alzheimer's Disease," J. Nut. Health and Aging 10: pp. 541-544
  • Tchantchou, F., Graves, M.V., Rogers, E., Ortiz, D., Shea, T.B. (2005) "N-acetyl cysteine alleviates oxidative damage to central nervous system of ApoE-deficient mice following folate and vitamin E-deficiency," Journal of Alzheimer's Disease 7:2 pp. 135-138
  • Lee, D.Y., Graves, M.V., Van Etten, J.L., Choi, . (2005) "T-J Functional Implication of the tRNA Genes Encoded in the Chlorella Virus PBCV-1 Genome," Plant Pathol J. 21: pp. 334-342
  • Tchantchou, F., Graves, M.V., Ortiz, D., Rogers, E., Shea, T.B. (2004) "Dietary supplementation with 3-deaza adenosine, N-acetyl cysteine, and S-adenosyl methionine provide neuroprotection against multiple consequences of vitamin deficiency and oxidative challenge. Relevance to age-related neurodegeneration," NeuroMolecular Medicine 6:2-3 pp. 93-104
  • Kang, M., Graves, M.V., Mehmel, M., Moran, A., Gazzarrini, A., Thiel, G., Gurnon, J.R., Van Etten, J.L. (2004) "Genetic Diversity in Chlorella Viruses Flanking KCV, a Gene that Encodes a Potassium Ion Channel Protein," Virology 356: pp. 150-159
  • Tchantchou, F., Graves, M.V., Ashline, D., Morin, A., Pimenta, A., Ortiz, D., Rogers, E., Shea, T.B. (2004) "Increased transcription and activity of glutathione synthase in response to deficiencies in folate, vitamin E, and apolipoprotein E," Journal of neuroscience research 75:4 pp. 508-15
  • Tchantchou, F., Graves, M.V., Ortiz, D., Rogers, E., Shea, T.B. (2004) "Interaction of folate deficiency and apolipoprotein E gene dosage on cognitive impairment and oxidative damage to brain tissue: Compensatory regulation of methionine cycle genes," Neurobiology of aging 25:
  • Van Etten, J.L., Graves, M.V., Mueller, D.G., Bolland, W., Delaroque, N. (2002) "Phycodnaviridae- Large DNA algal viruses," Archives in Virology 147: pp. 1479-1516
  • Nandhagopal, N., Simpson, A.A., Gurnon, J.R., Yan, X., Baker, T.S., Graves, M.V., Van Etten, J.L., Rossmann, M.G. (2002) "The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus," Proceedings of the National Academy of Sciences of the United States of America 99:23 pp. 14758-14763
  • Graves, M.V., Bernadt, C.T., Cerny, R., Van Etten, J.L. (2001) "Molecular and Genetic Evidence for a Virus-Encoded Glycosyltransferase involved in Protein Glycosylation," Virology 285: pp. 332-345
  • Sun, L., Gurnon, J.R., Kutish, G., Graves, M.V., Van Etten, J.L. (2000) "Characterization of a ß-1, 3-Glucanase Encoded by Chlorella Virus PBCV-1," Virology 276: pp. 27-36
  • Kaiser, A., Vollmert, M., Tholl, D., Graves, M.V., Gurnon, J.R., Xing, W., Lisec, A.D., Nickerson, K.W., Van Etten, J.L. (1999) "Chlorella virus PBCV-1 encodes a functional homospermidine synthase," Virology 263: pp. 254-262
  • Graves, M.V., Burbank, D.E., Roth, R., Heuser, J., DeAngelis, P.L., Van Etten, J.L. (1999) "Hyaluronan sythesis in virus PBCV-1 infected chlorella-like green algae," Virology 257:1 pp. 15-23
  • Landstein, D., Graves, M.V., Burbank, D.E., DeAngelis, P.L., Van Etten, J.L. (1998) "Chlorella virus PBCV-1 encodes functional glutamine: fructose-6-phosphate amidotransferase and UDP-glucose dehydrogenase enzymes," Virology 250: pp. 388-396
  • Graves, M.V., Pogany, J., Romero, J. (1997) "Defective interfering RNAs and defective viruses associated with multipartite RNA viruses of plants," Seminars in Virology 7: pp. 399-408
  • DeAngelis, P.L., Jing, W., Graves, M.V., Burbank, D.E., Van Etten, J.L. (1997) "Hyaluronan synthase of chlorella virus PBCV-1," Science 278: pp. 1800-1803
  • Graves, M.V., Roossinck, M.J. (1995) "Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus," Journnal of Virology 69: pp. 4746-4751
  • Graves, M.V., Meints, R.H. (1992) "Characterization of the gene encoding the most abundant in vitro translation product from virus-infected Chlorella-like algae," Gene 113: pp. 149-155
  • Graves, M.V., Meints, R.H. (1992) "Characterization of the major capsid protein and cloning of its gene from algal virus PBCV-1," Virology 188: pp. 198-207
  • McCluskey, K., Graves, M.V., Mills, D., Meints, R.H. (1992) "Replication of Chlorella virus PBCV-1 and host karyotype determination studied with pulse-field gel electrophoresis ," Journal of Phycology 28: pp. 846-850
  • Schuster, A.M., Graves, M.V., Korth, K., Zieglebein, M., Brumbaugh, J., Grone, D., Meints, R.H. (1990) "Transcription and sequence studies of a 4.3kbp fragment from a ds-DNA eukaryotic algal virus," Virology 176: pp. 515-523

Presentations

  • Are Viruses Alive? - General Virology lecture to sophomore biology students, 2012 - Kingston, NH
  • - Fundamentals in Biotechnology Workshop, 2011
  • Towards Predicting Protein-Protein Interactions in Novel Organisms - IEEE 7th International Conference on BioInformatics and BioEngineering, 2007 - Harvard Medical School, Boston, MA
  • Algal Virus Genomics - Inter-campus Marine Science Symposium, 2007 - Lowell, Massachusetts
  • Algal Virus Genomics - Microbial Genome Sequencing Annual Meeting, Plant & Animal Genome Conference, 2006 - San Diego, CA
  • Characterization of a Chlorella Virus Encoded Putative beta-lactamase Protein. - New England Biology Conference, 2006 - Boston, MA
  • Does PBCV-1 mRNA Contain a Translational Enhancer? - New England Biology Conference, 2006 - Boston, MA
  • Sequence Analysis of Two Large Chlorella Viruses - 4th Algal Virus Workshop, 2005 - Amsterdam, The Netherlands
  • Adaptations to host and transmission method in Plum pox potyvirus - American Society for Virology Annual Meeting, 2005 - Penn State University, University Park, PA
  • Introducing Freshman Biology Majors to Information Literacy - Conversations on Teaching and Learning: "Teaching with Technology", 2005
  • Genomic Comparisons of Chlorella Viruses to the 1.2 Megabase Mimivirus - Society for Virology Annual Meeting, 2005 - Penn State University, University Park, PA
  • Expression of recombinant A189/92 protein of PBCV-1 and the importance of tRNA genes encoded in the viral genome - 23rd American Society for Virology Meeting, 2004 - Montreal, Quebec, Canada
  • Folate deficiency alters expression of methionine cycle genes: Impact of apolipoprotein E gene dosage, 2004 - Boston, MA
  • Algal Virus Genomics - Microbial Genome Sequencing Program Awardees Workshop, 2004 - Arlington, VA
  • Functional Genmoics of a Virus: The Unusual Lifestyle of the Chloroviruses, 2003
  • Functional Genmoics of a Virus: The Unusual Lifestyle of the Chloroviruses - Biology Department Seminar, 2002 - Boston
  • Functional Genmoics of a Virus: The Unusual Lifestyle of the Chloroviruses - Environmental Biology Seminar, 2002
  • Functional Genmoics of a Virus: The Unusual Lifestyle of the Chloroviruses, 2002 - Indianapolis, IN
  • Structure of a large (T = 169) icosahedral dsDNA virus - 20th American Society for Virology Meeting, 2001 - Madison, WI
  • A PBCV-1-encoded glycosyltransferase involved in glycosylation of its major capsid protein - 19th American Society for Virology Meeting, 2000 - Ft. Collings, CO
  • Alteration of the Host Cell-Wall Surface During Chlorella Virus PBCV-1 Infection - 2nd Algal Virus Workshop, 2000 - Galway, Ireland
  • - Comparative Pathobiology / Plant Pathology Seminar, 2000
  • Genetic Analysis of Polyglycan Synthesis by Chlorella Virus PBCV-1: Chlorella Viruses, so sweet yet so misunderstood, 2000 - Bloomington, IN
  • Genetic Analysis of Polyglycan Synthesis by Chlorella Virus PBCV-1: Hyaluronan Synthesis and Protein Glycosylation, 2000 - Columbia, MO
  • Genetic Analysis of Polyglycan Synthesis by Chlorella Virus PBCV-1: Hyaluronan Synthesis and Protein Glycosylation, 2000 - Athens, GA
  • Viral encoded homospermidine synthesis in a Chlorella-like green algae - 18th American Society for Virology Meeting, 1999 - Amherst, MA
  • Structure and glycosylation of the major capsid protein of Chlorella-virus PBCV-1 - Plant Sciences Retreat, 1999
  • Hyaluronan synthesis in a virus infected algae - 17th American Society for Virology Meeting, 1998 - Vancouver, B.C., Canada
  • - Plant Biology Division 10th Anniversary Plant Biology Symposium, 1998 - Ardmore, OK
  • Chlorella virus PBCV-1 contains genes that encode functional proteins involved in sugar manipulation - 16th American Society for Virology Meeting, 1997 - Bozeman, MT
  • De novo generation of defective RNAs from cucumber mosaic virus - 15th American Society for Virology Meeting, 1996 - London, Ontario, Canada
  • , 1996 - DeKalb, IL
  • Host specific maintenance of a cucumovirus defective RNA - 14th American Society for Virology Meeting, 1995 - Austin, TX
  • Isolation and characterization of a new DI RNA derived from the Fny strain of cucumber mosaic cucumovirus - 13th American Society for Virology Meeting, 1994 - Madison, WI
  • Biochemical analysis of the replication of cucumber mosaic virus strains Fny and Sny in muskmelon - 12th American Society for Virology Meeting, 1993 - Davis, CA

Contracts, Fellowships, Grants and Sponsored Research

  • Algae Bioreactor Evaluation II (), Contract - Algae Grow Lab, Wakefield, MA
    Graves, M. (Principal)
  • Algal Bioreactor Evaluation I (), Contract - Algae Grow Lab, Wakefield, MA
    Graves, M. (Principal)
  • Chlorell spp. Genome Sequencing (), Grant - Joint Genome Institute, Department of Energy
    Graves, M.
  • Determining Protein-Protein Interactions in Virus Infected Chlorella-Like Green Algae (), Grant - UMass Lowell Seed Funding Program to Advance Research and Scholarship
    Graves, M.
  • Microeukaryote diversity in the Gulf of Maine: Under-explored microbial diversity in a well-studied coastal marine ecosystem (), Grant - International Census of Marine Sciences
    Graves, M. (Co-Principal)
  • Algae Photobioreactor Evaluation (2012), -
    Graves, M.V. (Principal)
  • Algae Photobioreactor Evaluation (2011), -
    Graves, M.V. (Principal)
  • Algal Virus Genomics (2003), Grant - NSF: Microbial Genome Sequencing Program
    Graves, M. (Principal)
  • Defective RNA Formation and Recombination in Bromoviruses (1995), - S. R. Noble Foundation Postdoctoral Excellence Award, Northern Illinois University
    Graves, M.