Two people working in the clean room at the Saab ETIC Building.

Amino Acid Sequence

  • NH2-terminal sequencing and peptide mapping/MS.

Post-Translational Modifications / Degradation

  • N-glycan profiling, peptide mapping/MS (glycosylation, glycation, phosphorylation, sulfation, deamidation and oxidation), ion exchange-HPLC (sialylation, sulfation and deamidation) and Ellman’s assay (unpaired Cys residues).


  • SDS-PAGE, Western blot, size exclusion-HPLC, reversed phase-HPLC.

Aggregation/Structural Stability

  • Near- and far-UV CD, intrinsic Trp fluorescence, structural melting curve, limited protease cleavage, multi-angle laser light scattering (MALLS), size exclusion-HPLC, SDS-PAGE. 


  • In vitro binding by ELISA and Biacore.

Production Titer

  • Protein A-HPLC for Abs and Fc-fusion proteins, product-specific ELISA and reversed phase-HPLC.