Matthew Nugent

Matthew A. Nugent, Ph.D.

Associate Dean for Research, Innovation and Partnerships, Professor

College
College of Sciences
Department
Biological Science, SCORE
Phone
978-934-2888
Fax
978-934-3044
Office
Olney Hall - 524
Email
matthew_nugent@uml.edu
Links

Expertise

Cell Biology & Biochemistry; Extracellular Matrix; Growth Factors; Proteoglycans; Vascular Biology

Research Interests

Extracellular Matrix; Cardiovascular Disease; Cancer; Cell Biology; Growth Factors; Enzymology; Lung Disease; COPD.

Cells within multicellular organisms are in constant communication with one another in order to coordinate tissue, organ and organism-wide responses to changing conditions. Intercellular communication is principally mediated through the extracellular space, and as such, transport and interaction of signaling molecules within the extracellular matrix represents a major point of control in biology. Not surprisingly, disruptions in cell-extracellular matrix communication invariably lead to disease.  Our lab has focused on how the composition and mechanical state of the extracellular matrix control protein growth factors. We are particularly interested in how the complex polysaccharide heparan sulfate controls growth factor storage, release and activity within the extracellular space.  We study the mechanisms by which structural heterogeneities within heparan sulfate mediate specificity of growth factor binding and activity. We apply a combination of biochemical, molecular, biophysical, and computational approaches in conjunction with cell culture and animal studies to generate a systems biology view of growth factor regulation that incorporates the influence of multiple factors on one another. We apply this approach to studies aimed at understanding the details of growth factor-receptor recognition and activation, the regulation of angiogenesis, and the control of extracellular matrix turnover. The overriding theme to our research is to use quantitative methods to analyze complex biological processes in order to develop predictive models of living systems that can be used to probe basic mechanisms and to assist in the rational design of new therapies for human disease. Currently we are focusing on the involvement of heparan sulfate proteoglycans, vascular endothelial growth factor, fibroblast growth factors, fibronectin, and neutrophil elastase in cardiovascular disease, chronic obstructive pulmonary disease, and retinopathies.

Major outstanding questions:

  1. What controls the structural complexity of heparan sulfate?
  2. How are growth factor specificity and sensitivity modulated by the state of the extracellular matrix?
  3. How is extracellular matrix turnover in response to injury controlled in order to maintain structural and functional tissue homeostasis?
  4. What catalytic processes modify extracellular matrix structure and function?

Education

  • Other: Chemical Engineering, (1992), Massachusetts Institute of Technology - Cambridge, MA
    Supporting Area: Health Sciences and Technology
    Dissertation/Thesis Title: Growth factor binding to heparan sulfate
  • Ph D: Graduate Department of Biochemistry, (1990), Brandeis University
    Dissertation/Thesis Title: Mechanisms of growth regulation by transforming growth factor-beta. Thesis advisor: Dr. Michael Newman
  • BA: Biochemistry, (1983), Brandeis University

Biosketch

Mathew Nugent is the Associate Dean for Research, Innovation and Partnerships at the Kennedy College of Sciences and is Professor of the Department of Biological Sciences at UMass Lowell.

Before joining UMass Lowell he was a Professor of Biochemistry, Biomedical Engineering and Ophthalmology at Boston University.

Selected Awards and Honors

  • Wong Research Fund Award (2015), Scholarship/Research - Kennedy College of Sciences, UMass Lowell
  • Educator of the Year, Graduate Medical Sciences, Boston University School of Medicine (2013)
  • Selected as a delegate to participate in the first Indo-US Frontiers of Engineering Forum, Agra, India, by the National Academy of Engineering (2006)
  • Gordon Conference on Proteoglycans –Best Presentation Award (2002)
  • American Cancer Society, Research Award (1994), Scholarship/Research - American Cancer Society
  • Marquis Who's Who in Science and Engineering 2nd Edition (1994) - Marquis
  • Whitaker Foundation Biomedical Engineering Research Award (1994), Scholarship/Research - Whitaker Foundation
  • American Heart Association, Grant-In-Aid Award (1993)
  • Eye Bank Association of America, Scientific Research Prize (1993)
  • Fight For Sight, Research Award (1993), Scholarship/Research
  • NIH National Research Service Award, Individual Postdoctoral Fellowship, MIT (1990), Scholarship/Research - NIH, MIT
  • Hoffman-LaRoche Graduate Research Fellowship (1987), Leadership - Roche Institute of Mol. Biol.
  • NIH Public Health Service Fellowship (1984), Scholarship/Research - NIH, Brandeis University
  • Dean's List (1983) - Brandeis University
  • Earthwatch Research Scholarship (1980), Scholarship/Research - Pleasant Bay Marine Biol. Lab., Orleans, MA
  • Bausch and Lomb Award for Scientific Achievement (1979), Scholarship/Research - Bausch and Lomb
  • Earthwatch Research Scholarship (1979), Scholarship/Research
  • Rensselaer Polytechnic Institute Math and Science Medal and Scholarship (1978), Scholarship/Research - Rensselaer Polytechnic Institute

Selected Publications

  • Hubbard, B., Buczek-Thomas, J.A., Nugent, M.A., Smith, M.L. (2016). Fibronectin Fiber Extension Decreases Cell Spreading and Migration. Journal of Cellular Physiology, 231(8) 1728 - 1736.
  • Derricks, K.E., Trinkaus-Randall, V., Nugent, M.A. (2015). Extracellular Matrix Stiffness Modulates VEGF Calcium Signaling in Endothelial Cells: Individual Cell and Population Analysis. Integrative Biol, 7 1011-1025.
  • Kurtagic, E., Rich, C.B., Buczek-Thomas, J., Nugent, M.A. (2015). Neutrophil Elastase-Generated Fragment of Vascular Endothelial Growth Factor-A Stimulates Macrophage and Endothelial Progenitor Cell Migration. PLoS ONE, 10(12) 1-17.
  • Teran, M., Nugent, M.A. (2015). Synergistic Binding of Vascular Endothelial Growth Factor-A and Its Receptors to Heparin Selectively Modulates Complex Affinity. The Journal Of Biological Chemistry, 290(26) 16451-16462.
  • Hubbard, B., Buczek-Thomas, J., Nugent, M.A., Smith, M.L. (2015). Fibronectin Fiber Extension Decreases Cell Spreading and Migration. J Cell Physiol.
  • Huang, Y., Mao, Y., Buczek-Thomas, J., Nugent, M.A., Zaia, J. (2014). Oligosaccharide Substrate Preferences of Human Extracellular Sulfatase Sulf2 Using Liquid Chromatography-Mass Spectrometry Based Glycomics Approaches. PLoS One 9.
  • Sazonova, O.V., Isenberg, B.C., Herrmann, J., Lee, K.L., Purwada, A., Valentine, A.D., Buczek-Thomas, J.A., Wong, J.Y., Nugent, M.A. (2014). Extracellular Matrix Presentation Modulates Vascular Smooth Muscle Cell Mechanotransduction. Matrix biology : journal of the International Society for Matrix Biology.
  • Mao, Y., , Buczek-Thomas, J.A., Ethen, C.M., Nugent, M.A., Wu, Z.L., Zaia, J. (2014). A Liquid Chromatography-Mass Spectrometry Based Approach to Characterize the Substrate Specificity of Mammalian Heparanase. The Journal of Biological Chemistry.
  • Mottarella, S., Beglov, D., Beglova, N., Nugent, M.A., Kozakov, D., Vajda, S. (2014). Docking Server for the Identification of Heparin Binding Sites on Proteins. Journal of Chemical Information and Modeling, 54(7) 2068-78.
  • Lee, A., Derricks, K., Minns, M., Ji, S., Chi, C., Nugent, M.A., Trinkaus-Randall, V. (2014). Hypoxia-Induced Changes in Ca(2+) Mobilization and Protein Phosphorylation Implicated in Impaired Wound Healing. American journal of physiology. Cell physiology, 306(10) C972-85.
  • Hubbard, B., Buczek-Thomas, J.A., Nugent, M.A., Smith, M.L. (2014). Heparin-Dependent Regulation of Fibronectin Matrix Conformation. Matrix Biology, 34 124-31.
  • Tumelty , K.E., Nugent, M.A., Smith, B.D., Layne, M.A. (2014). Aortic Carboxypeptidase-Like Protein (ACLP) Enhances Lung Myofibroblast Differentiation Through Transforming Growth Factor β Receptor-Dependent and -Independent Pathways. Journal of Biological Chemistry, 289(5) 2526-2536.
  • Huang, Y., Mao, Y., Buczek-Thomas, J.A., Nugent, M.A., Zaia, J. (2014). Oligosaccharide Substrate Preferences of Human Extracellular Sulfatase sulf2 Using Liquid Chromatography-Mass Spectrometry Based Glycomics Approaches. PloS one, 9(8) e105143.
  • Derricks , K.E., Rich , C.B., Buczek-Thomas , J.a., Nugent , M.A. (2013). Ascorbate Enhances Elastin Synthesis in 3D Tissue-Engineered Pulmonary Fibroblasts Constructs. Tissue and Cell, 45(4) 253-260.
  • Nugent, M.A., Zaia, J., Spencer, J.L., . (2013). Heparan Sulfate-Protein Binding Specificity. Biochemistry (Moscow), 78(7) 726-735.
  • Imsirovic, J., Derricks, K., Buczek-Thomas, J.A., Rich, C.B., Nugent, M.A., Suki, B. (2013). A Novel Device to Stretch Multiple Tissue Samples with Variable Patterns: Application for mRNA Regulation in Tissue-Engineered Constructs. Biomatter, 3(3).
  • Fannon, M., Parekh, E., Buczek-Thomas, J., Forsten-Williams, K., Bach, B., Zhao, B., Chu, P., Goerges-Wildt, ., Nugent, M. (2012). Facilitated Diffusion of VEGF165 Through Descemet’s Membrane with Sucrose Octasulfate. Journal of Cellular Physiology, 227(11) 3693-3700.
  • Bais, M., Kirsch, K., Nugent, M., Stephens, D., Sume, S., Trackman, P., Sonenshein, G. (2012). Recombinant Lysyl Oxidase Propeptide Protein Inhibits Growth and Promotes Apoptosis of Pre-Existing Murine Breast Cancer Xenografts. PLoS ONE, 7(2).
  • Sazonova, O., Nugent, M., Lee, K., Isenberg, B., Rich, C., Wong, J. (2011). Cell-Cell Interactions Mediate the Response of Vascular Smooth Muscle Cells to Substrate Stiffness. Biophysical Journal, 101(3) 622-630.
  • Forsten-Williams , K., Kurtagic , E., Nugent, M. (2011). Complex Receptor-Ligand Dynamics Control the Response of the VEGF System to Protease Injury. BMC Systems Biology, 5.
  • Araújo, A., Spencer, J., Majumdar, A., Parameswaran, H., Yi, E., Nugent , M., Suki, B. (2011). Dynamics of Enzymatic Digestion of Elastic Fibers and Networks Under Tension. Proceedings of the National Academy of Sciences of the United States of America, 108(23) 9414-9419.
  • Naimy, H., Zaia, J., Buczek-Thomas, J., Nugent, M., Leymarie, N. (2011). Highly Sulfated Nonreducing End-Derived Heparan Sulfate Domains Bind Fibroblast Growth Factor-2 with High Affinity and are Enriched in Biologically Active Fractions. Journal of Biological Chemistry, 286(22) 19311-19319.
  • Spencer, J., Nugent, M., Bernanke, J., Buczek-Thomas, J. (2010). A Computational Approach for Deciphering the Organization of Glycosaminoglycans. PLoS ONE, 5(2).
  • Guarnieri, F., Stone, P., Nugent, M., Spencer, J., Lucey, E. (2010). A Human Surfactant Peptide-Elastase Inhibitor Construct as a Treatment for Emphysema. Proceedings of the National Academy of Sciences of the United States of America, 107(23) 10661-10666.
  • Vora, S., Pischon, N., Palamakumbura, A., Mitsi, M., Guo, Y., Nugent, M., Trackman, P. (2010). Lysyl Oxidase Propeptide Inhibits FGF-2-Induced Signaling and Proliferation of Osteoblasts. Journal of Biological Chemistry, 285(10) 7384-7393.
  • Symes, K., Nugent, M., Smith, E., Mitsi, M. (2010). Sweet Cues: How Heparan Sulfate Modification of Fibronectin Enables Growth Factor Guided Migration of Embryonic Cells. Cell Adhesion and Migration, 4(4) 507-510.
  • Ritter , M., Buczek-Thomas, J., Suki, B., Jesudason, R., Majumdar, A., Stamenović, D., Stone, P., Nugent, M. (2009). A Zipper Network Model of the Failure Mechanics of Extracellular Matrices. Proceedings of the National Academy of Sciences of the United States of America, 106(4) 1081-1086.
  • Palamakumbura, A., Sonenshein, G., Vora, S., Nugent, M., Kirsch, K., Trackman, P. (2009). Lysyl Oxidase Propeptide Inhibits Prostate Cancer Cell Growth by Mechanisms That Target FGF-2-Cell Binding and Signaling. Oncogene, 28(38) 3390-3400.
  • Kurtagic, E., Jedrychowski, M.P., Nugent, M.A. (2009). Neutrophil Elastase Cleaves VEGF to Generate a VEGF Fragment with Altered Activity. American Journal of Physiology - Lung Cellular and Molecular Physiology, 296(3) L534-L546.
  • Smith, E.M., Symes, M., Nugent, M.A., Misti, M. (2009). PDGF-A Interactions with Fibronectin Reveal a Critical Role for Heparan Sulfate in Directed Cell Migration During Xenopus Gastrulation. Proceedings of the National Academy of Sciences of the United States of America, 106(51) 21683-21688.
  • Mitsi, M., Forsten-Williams, K., Nugent, M.A., Gopalakrishnan , M. (2008). A Catalytic Role of Heparin Within the Extracellular Matrix. Journal of Biological Chemistry, 283(50) 34796-34807.
  • Forsten-Williams, K., Nugent, M.A., Buczek-Thomas, J.A., Chu , C.L., Fannon, M. (2008). Control of Growth Factor Networks by Heparan Sulfate Proteoglycans. Annals of Biomedical Engineering, 36(12) 2134-2148.
  • Yang, S., Nugent , M.A., Panchenko, M.P. (2008). EGF Antagonizes TGF-β-Induced Tropoelastin Expression in Lung Fibroblasts via Stabilization of Smad Corepressor TGIF. American Journal of Physiology - Lung Cellular and Molecular Physiology, 295(1) L143-L151.
  • Baker, A.B., Iozzo, R.V., Nugent, M.A., Ettenson , D.S., Jonas, M., Edelman, E.R. (2008). Endothelial Cells Provide Feedback Control for Vascular Remodeling Through a Mechanosensitive Autocrine TGF-β Signaling Pathway. Circulation Research, 103(3) 289-297.
  • Buczek-Thomas , J.A., Nugent , M.A., Foster, J.A., Hsia, E., Rich, C.B. (2008). Inhibition of Histone Acetyltransferase by Glycosaminoglycans. Journal of Cellular Biochemistry, 105(1) 108-120.
  • Fannon, M., Chia, L.C., Kaipainen, A., Gregory, K.J., Nugent, M.A., Forsten-Williams, K., Barnes, C., Lapp, C., Shing, Y., Goerges-Wildt, A.L., Panigrahy, D. (2008). Sucrose Octasulfate Regulates Fibroblast Growth Factor-2 Binding, Transport, and Activity: Potential for Regulation of Tumor Growth. Journal of Cellular Physiology, 215(2) 434-441.
  • Suki, B., Bates, J.H., Nugent, M.A., Majumdar, A. (2007). In Silico Modeling of Interstitial Lung Mechanics: Implications for Disease Development and Repair. Drug Discovery Today: Disease Models, 4(3) 139-145.
  • Mitsi , M., Nugent, M.A., Hong, Z., Costello , C.E. (2006). Heparin-Mediated Conformational Changes in Fibronectin Expose Vascular Endothelial Growth Factor Binding Sites. Biochemistry, 45(34) 10319-10328.
  • DiCamillo, S.J., Naggar, E.F., Nugent, M.A., Panchenko, M.V., Yang, S., Panchenko, M.P., Toselli, N.A., Rich , C.B., Stone, P.J. (2006). Neutrophil Elastase-Initiated EGFR/MEK/ERK Signaling Counteracts Stabilizing Effect of Autocrine TGF-β on Tropoelastin MRNA in Lung Fibroblasts. American Journal of Physiology - Lung Cellular and Molecular Physiology, 291(2) L232-L243.
  • Spencer, J.L., Stone, P.J., Nugent, M.A. (2006). New Insights into the Inhibition of Human Neutrophil Elastase by Heparin. Biochemistry, 45(30) 9104-9120.
  • Gopalakrishnan , M., Forsten-Williams , K., Nugent , M.A., Täuber , U.C. (2005). Effects of Receptor Clustering on Ligand Dissociation Kinetics: Theory and Simulations. Biophysical Journal, 89(6) 3686-3700.
  • Chu, C.L., Goerges, A.L., Nu, M.A. (2005). Identification of Common and Specific Growth Factor Binding Sites in Heparan Sulfate Proteoglycans. Biochemistry, 44(36) 12203-12213.
  • Nugent, M.A., Forsten-Williams, K., Karnovsky, M.J., Edelman, E.R. (2005). Mechanisms of Cell Growth Regulation by Heparin and Heparan Sulfate (pp. 533-570). Chemistry and Biology of Heparin and Heparan Sulfate
  • Forsten-Williams, K., Chua, C.C., Nugent, M.A. (2005). The Kinetics of FGF-2 Binding to Heparan Sulfate Proteoglycans and MAP Kinase Signaling. Journal of Theoretical Biology, 233(4) 483-499.
  • Buczek-Thomas , J.A., Lucey, E.C., Nugent , M.A., Stone, P.J., Chu, C.L., Rich, C.B., Carreras, I., Goldstein, R.H., Foster , J.A. (2004). Elastase Mediates the Release of Growth Factors from Lung in Vivo. American Journal of Respiratory Cell and Molecular Biology, 31(3) 344-350.
  • Chu , C.L., Nugent , M.A., Buczek-Thomas , J.A. (2004). Erratum: Heparan Sulphate Proteoglycans Modulate Fibroblast Growth Factor-2 Binding Through a Lipid Raft-Mediated Mechanism (Biochemical Journal (2004) 379 (331-341)). Biochemical Journal, 380(3) 951.
  • Chua , C.C., Nugent , M.A., Forsten-Williams , K., Rahimi, N. (2004). Heparan Sulfate Proteoglycans Function as Receptors for Fibroblast Growth Factor-2 Activation of Extracellular Signal-Regulated Kinases 1 and 2. Circulation Research, 94(3) 316-323.
  • Chu, C.L., Nugent, M.A., Buczek-Thomas, J.A. (2004). Heparan Sulphate Proteoglycans Modulate Fibroblast Growth Factor-2 Binding Through a Lipid Raft-Mediated Mechanism. Biochemical Journal, 379(2) 331-341.
  • Goerges, A.L., Nugent, M.A. (2004). pH Regulates Vascular Endothelial Growth Factor Binding to Fibronectin: A Mechanism for Control of Extracellular Matrix Storage and Release. Journal of Biological Chemistry, 279(3) 2307-2315.
  • Fannon, M., Forsten-Williams, K., Dowd, C.J., Freedman, D.A., Folkman , J., Nugent , M.A. (2003). Binding Inhibition of Angiogenic Factors by Heparan Sulfate Proteoglycans in Aqueous Humor: Potential Mechanism for Maintenance of an Avascular Environment. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 17(8) 902-904.
  • Izvolsky, K.I., Shoykhet, D., Yang, Y., Yu , Q., Nugent , M.A., Cardoso, W.V. (2003). Heparan Sulfate-FGF10 Interactions During Lung Morphogenesis. Developmental Biology, 258(1) 185-200.
  • Izvolsky, K.I., Zhong, L., Wei, L., Nugent, M.A., Cardoso, W.V., Yu , Q. (2003). Heparan Sulfates Expressed in the Distal Lung Are Required for FGF10 Binding to the Epithelium and for Airway Branching. American Journal of Physiology - Lung Cellular and Molecular Physiology, 285(4 29-4) L838-L846.
  • Liu, J., Rich, C.B., Buczek-Thomas , J.A., Nugent, M.A., Panchenko, M.P., Foster, J.A. (2003). Heparin-Binding EGF-Like Growth Factor Regulates Elastin and FGF-2 Expression in Pulmonary Fibroblasts. American Journal of Physiology - Lung Cellular and Molecular Physiology, 285(5 29-5) L1106-L1115.
  • Li , W., Nugent , M.A., Kagan, H.M., Zhao , Y., Chau , A.N., Li , S.J., Chou , I., Liu , G. (2003). Lysyl Oxidase Oxidizes Basic Fibroblast Growth Factor and Inactivates Its Mitogenic Potential. Journal of Cellular Biochemistry, 88(1) 152-164.
  • Hsia , E., Richardson , T.P., Nugent, M.A. (2003). Nuclear Localization of Basic Fibroblast Growth Factor Is Mediated by Heparan Sulfate Proteoglycans Through Protein Kinase C Signaling. Journal of Cellular Biochemistry, 88(6) 1214-1225.
  • Goerges, A.L., Nugent , M.A. (2003). Regulation of Vascular Endothelial Growth Factor Binding and Activity by Extracellular pH. Journal of Biological Chemistry, 278(21) 19518-19525.
  • Rich , C.B., Carreras , I., Foster, J.A., Lucey , E.C., Jaworski , J.A., Buczek-Thomas, J.A., , J.A., Nugent , M.A., Stone , P. (2003). Transcriptional Regulation of Pulmonary Elastin Gene Expression in Elastase-Induced Injury. American Journal of Physiology - Lung Cellular and Molecular Physiology, 285(2 29-2) L354-L362.
  • Dicamillo , S.J., Carreras, I., Panchenko , M.V., Stone , P.J., Nugent , M.A., Foster , J.A., Panchenko, M.P. (2002). Elastase-Released Epidermal Growth Factor Recruits Epidermal Growth Factor Receptor and Extracellular Signal-Regulated Kinases to Down-Regulate Tropoelastin mRNA in Lung Fibroblasts. Journal of Biological Chemistry, 277(21) 18938-18946.
  • Brown, C.T., Lin , P., Walsh , M.T., Gantz , D., Nugent , M.A., Trinkaus-Randall , V. (2002). Extraction and Purification of Decorin from Corneal Stroma Retain Structure and Biological Activity. Protein Expression and Purification, 25(3) 389-399.
  • Buczek-Thomas , J.A., Chu , C.L., Rich, C.B., Stone , P.J., Nugent , M.A., Foster , J.A. (2002). Heparan Sulfate Depletion Within Pulmonary Fibroblasts: Implications for Elastogenesis and Repair. Journal of Cellular Physiology, 192(3) 294-303.
  • Song , Q.H., Klepeis, V.E., Nugent, M.A., Trinkaus-Randall, V. (2002). TGF-β1 Regulates TGF-β1 and FGF-2 mRNA Expression During Fibroblast Wound Healing. Journal of Clinical Pathology - Molecular Pathology, 55(3) 164-176.
  • Forsten , K.E., Nugent , M.A. (2001). Binding Constant Measurements for Inhibitors of Growth Factor Binding to Heparan Sulfate Proteoglycans. Methods in molecular biology (Clifton, N.J.), 171 415-426.
  • Richardson , T.P., Trinkaus-Randal , V., Nugent , M.A. (2001). Regulation of Heparan Sulfate Proteoglycan Nuclear Localization by Fibronectin. Journal of Cell Science, 114(9) 1613-1623.
  • Nugent , M.A., Iozzo, R.V. (2000). Fibroblast Growth Factor-2. International Journal of Biochemistry and Cell Biology, 32(2) 115-120.
  • Consigny , P.M., Nugent , M.A., Lambert , C.R., Kandarpa , K., Machan , L., Raper , S., Brenner , M.K., Blau , H.M., Crystal , R.G., Robinson , D.M., Bailey , S.R., Haskal , Z.J., Dake , M.D., Rajagopolan , S., Hustinx , R., Eck, S.L., Alavi , A., Vincent, K.A., Shyu , K., Luo , Y., Magner , M., Tio, R.A., Jiang , C., Goldberg , M.A., Akita , G.Y., Isner , J.M., Gregory , R.J., Barry , J.J. (2000). Gene Therapy and Local Drug Delivery. Journal of Vascular and Interventional Radiology, 11(2 II) 409-444.
  • Nugent, M. (2000). Heparin Sequencing Brings Structure to the Function of Complex Oligosaccharides. Proceedings of the National Academy of Sciences of the United States of America, 97(19) 10301-10303.
  • Sperinde, G.V., Nugent , M.A. (2000). Mechanisms of Fibroblast Growth Factor 2, Intracellular Processing: A Kinetic Analysis of the Role of Heparan Sulfate Proteoglycans. Biochemistry, 39(13) 3788-3796.
  • Nugent , M.A., Nugent , H.M., Iozzo , R.V., Sanchack , K., Edelman , E.R. (2000). Perlecan is Required to Inhibit Thrombosis After Deep Vascular Injury and Contributes to Endothelial Cell-Mediated Inhibition of Intimal Hyperplasia. Proceedings of the National Academy of Sciences of the United States of America, 97(12) 6722-6727.
  • Forsten , K.E., Nugent , M.A., Fannon , M. (2000). Potential Mechanisms for the Regulation of Growth Factor Binding by Heparin. Journal of Theoretical Biology, 205(2) 215-230.
  • Fannon , M., Forsten , K.E., Nugent , M.A. (2000). Potentiation and Inhibition of bFGF Binding by Heparin: A Model for Regulation of Cellular Response. Biochemistry, 39(6) 1434-1445.
  • Forsten , K.e., Wang , N., Robinson , R.M., Nugent , M.A. (2000). Simple Assay for Evaluating Inhibitors of Proteoglycan-Ligand Binding. Annals of Biomedical Engineering, 28(1) 119-127.
  • Csordas, G., Santra, M., Reed, C., Eichstetter, I., McQuillan, D., Gross, D., Nugent, M. (2000). Sustained Down-Regulation of the Epidermal Growth Factor Receptor by Decorin a Mechanism for Controlling Tumor Growth in Vivo. Journal of Biological Chemistry, 275((42)) 32879-32887.
  • Song , Q.H., Singh , R.P., Richardson , T.P., Nugent , M.A., Trinkaus-Randall , V. (2000). Transforming Growth Factor-β1 Expression in Cultured Corneal Fibroblasts in Response to Injury. Journal of Cellular Biochemistry, 77(2) 186-199.
  • Rich , C.B., Fontanilla , M.R., Nugent , M., Foster , J.A. (1999). Basic Fibroblast Growth Factor Decreases Elastin Gene Transcription Through an AP1/cAMP-Response Element Hybrid Site in the Distal Promoter. Journal of Biological Chemistry, 274(47) 33433-33439.
  • Brown , C.T., Nugent , M.A., Lau , F.W., Trinkaus-Randall , V. (1999). Characterization of Proteoglycans Synthesized by Cultured Corneal Fibroblasts in Response to Transforming Growth Factor β and Fetal Calf Serum. Journal of Biological Chemistry, 274(11) 7111-7119.
  • Buczek-Thomas , J.A., Nugent , M.A. (1999). Elastase-Mediated Release of Heparan Sulfate Proteoglycans from Pulmonary Fibroblast Cultures. a Mechanism for Basic Fibroblast Growth Factor (bFGF) Release and Attenuation of bFGF Binding Following Elastase-Induced Injury. Journal of Biological Chemistry, 274(35) 25167-25172.
  • Dowd , C.J., Cooney , C.L., Nugent , M.A. (1999). Heparan Sulfate Mediates bFGF Transport Through Basement Membrane by Diffusion with Rapid Reversible Binding. Journal of Biological Chemistry, 274(8) 5236-5244.
  • Natke , B., Venkataraman , G., Nugent , M.A., Sasisekharan , R. (1999). Heparinase Treatment of Bovine Smooth Muscle Cells Inhibits Fibroblast Growth Factor-2 Binding to Fibroblast Growth Factor Receptor but Not FGF-2 Mediated Cellular Proliferation. Angiogenesis, 3(3) 249-257.
  • Richardson , T.P., Trinkaus-Randall , V., Nugent , M.A. (1999). Regulation of Basic Fibroblast Growth Factor Binding and Activity by Cell Density and Heparan Sulfate. Journal of Biological Chemistry, 274(19) 13534-13540.
  • Nehra , A., Gettman , M.T., Moreland, R.B., Nugent , M., Bostwick, D.G., Barrett , D.M., Goldstein , I., Krane , R.J. (1999). Transforming Growth Factor-β1 (TGF-β1) Is Sufficient to Induce Fibrosis of Rabbit Corpus Cavernosum in Vivo. Journal of Urology, 162(3 I) 910-915.
  • Sharma, B., Handler , M., Eichstetter , I., Whitelock , J.M., Nugent , M.A., Iozzo , R.V. (1998). Antisense Targeting of Perlecan Blocks Tumor Growth and Angiogenesis in Vivo. Journal of Clinical Investigation, 102(8) 1599-1608.
  • Kypreos , K.E., Nugent , M.A., Sonenshein , G.E. (1998). Basic Fibroblast Growth Factor-Induced Decrease in Type I Collagen Gene Transcription is Mediated by B-myb. Cell Growth and Differentiation, 9(9) 723-730.
  • Trinkaus-Randall, V., Nugent , M.A. (1998). Biological Response to a Synthetic Cornea. Journal of Controlled Release, 53(1-3) 205-214.
  • Carreras , I., Rich , C.B., Stone , P.J., Lucey , E.C., Nugent , M.A., Foster , J.A. (1998). Development of a New in Vivo Model of Pulmonary Emphysema. FASEB Journal, 12(5).
  • Buczek-Thomas , J., Nugent , M.A. (1998). Effect of Elastase on bFGF and bFGF Receptor Function in Neonatal Rat Lung Fibroblast Matrices. FASEB Journal, 12(5).
  • Sperinde, G.V., Nugent, M.A. (1998). Heparan Sulfate Proteoglycans Control Intracellular Processing of bFGF in Vascular Smooth Muscle Cells. Biochemistry, 37(38) 13153-13164.
  • Forsten , K.E., Courant , N.A., Nugent , M.A. (1997). Endothelial Proteoglycans Inhibit bFGF Binding and Mitogenesis. Journal of Cellular Physiology, 172(2) 209-220.
  • Lau , F., Nugent , M., Trinkaus-Randall, V. (1997). Synthesis of Proteoglycans in the Presence of tgf-β by Cultured Stromal Fibroblasts. Investigative Ophthalmology and Visual Science, 38(4).
  • Fannon , M., Nugent , M.A. (1996). Basic Fibroblast Growth Factor Binds Its Receptors, Is Internalized, and Stimulates Dna Synthesis in Balb/c3T3 Cells in the Absence of Heparan Sulfate. Journal of Biological Chemistry, 271(30) 17949-17956.
  • Rich , C.B., Nugent, M.A., Stone , P., Foster , J.A. (1996). Elastase Release of Basic Fibroblast Growth Factor in Pulmonary Fibroblast Cultures Results in Down-Regulation of Elastin Gene Transcription. a Role for Basic Fibroblast Growth Factor in Regulating Lung Repair. Journal of Biological Chemistry, 271(38) 23043-23048.
  • Nugent, M.A., Dowd , C.J., Ryan, W.J., Courant , N.A., Forsten , K.E. (1996). Endothelial Cell Proteoglycans Inhibit bFGF Binding, Mitogenesis and Angiogenesis. Investigative Ophthalmology and Visual Science, 37(3).
  • Brown , C.T., Nugent , M.A., Trinkaus-Randal , V. (1996). In Vitro Synthesis of Proteo Glycans by Stromal Fibroblasts in the Absence of Platelet Associated Growth Factors. Investigative Ophthalmology and Visual Science, 37(3).
  • Nehra, A., Goldstein, I., Pabby, A., Nugent, M., Huang, Y.H., De Las Morenas, A., Krane, R.J., Udelson , D., De Tejada, I.S., Moreland , R.B. (1996). Mechanisms of Venous Leakage: A Prospective Clinicopathological Correlation of Corporeal Function and Structure. Journal of Urology, 156(4) 1320-1329.
  • Nathan , A., Nugent , M.A., Edelman , E.R. (1995). Tissue Engineered Perivascular Endothelial Cell Implants Regulate Vascular Injury. Proceedings of the National Academy of Sciences of the United States of America, 92(18) 8130-8134.
  • Hobbs , S.K., Peroilat , L.M., Cima , L.G., Nugent, M., Leunig , M., Jain, R.K. (1994). Development of an in Situ Assay for bFGF Release (331: pp. 181-192). Materials Research Society Symposium Proceedings
  • Sasisekharan, R., Moses , M.A., Nugent , M.A., Cooney , C.L., Langer , R. (1994). Heparinase Inhibits Neovascularization. Proceedings of the National Academy of Sciences of the United States of America, 91(4) 1524-1528.
  • Edelman , E.R., Nugent , M.A., Karnovsky , M.J. (1993). Perivascular and Intravenous Administration of Basic Fibroblast Growth Factor: Vascular and Solid Organ Deposition. Proceedings of the National Academy of Sciences of the United States of America, 90(4) 1513-1517.
  • Nugent , M.A., Karnovsky , M.J., Edelman , E.R. (1993). Vascular Cell-Derived Heparan Sulfate Shows Coupled Inhibition of Basic Fibroblast Growth Factor Binding and Mitogenesis in Vascular Smooth Muscle Cells. Circulation Research, 73(6) 1051-1060.
  • Edelman , E.R., Nugent , M.A., Smith , L.T., Karnovsky, M.J. (1992). Basic Fibroblast Growth Factor Enhances the Coupling of Intimal Hyperplasia and Proliferation of Vasa Vasorum in Injured Rat Arteries. Journal of Clinical Investigation, 89(2) 465-473.
  • Nugent , M.A., Edelman , E.R. (1992). Kinetics of Basic Fibroblast Growth Factor Binding to Its Receptor and Heparan Sulfate Proteoglycan: A Mechanism for Cooperatively. Biochemistry, 31(37) 8876-8883.
  • Nugent , M.A., Edelman , E.R. (1992). Transforming Growth Factor β1 Stimulates the Production of Basic Fibroblast Growth Factor Binding Proteoglycans in Balb/c3T3 Cells. Journal of Biological Chemistry, 267(29) 21256-21264.
  • Edelman , E.R., Nugent , M.A. (1991). Controlled Release of Basic Fibroblast Growth Factor. Drug News and Perspectives, 4(6) 352-357.
  • Newman , M.J., Lane , E.A., Iannotti , A.M., Nugent , M.A., Pepinsky , R.B., Keski-Oja , J. (1990). Characterization and Purification of a Secreted Plasminogen Activator Inhibitor (Pai-1) Induced by Transforming Growth Factor-β1 in Normal Rat Kidney (NRK) Cells: Decreased PAI-1 Expression in Transformed NRK Cells. Endocrinology, 126(6) 2936-2946.
  • Nugent , M.A., Lane , E.A., Keski-Oja , J., Moses , H.L., Newman , M.J. (1989). Growth Stimulation, Altered Regulation of Epidermal Growth Factor Receptors, and Autocrine Transformation of Spontaneously Transformed Normal Rat Kidney Cells by Transforming Growth Factor Beta. Cancer Research, 49(14) 3884-3890.
  • Nugent , M.A., Newman , M.J. (1989). Inhibition of Normal Rat Kidney Cell Growth by Transforming Growth Factor-β Is Mediated by Collagen. Journal of Biological Chemistry, 264(30) 18060-18067.
  • Biswas , C., Nugent , M.A. (1987). Membrane Association of Collagenase Stimulatory Factor(s) from B-16 Melanoma Cells. Journal of cellular biochemistry, 35(3) 247-258.
  • Newman , M.J., Lane , E.A., Nugent , M.A., Racker , E. (1986). Induction of Anchorage-Independent Growth by Epidermal Growth Factor and Altered Sensitivity to Type β-Transforming Growth Factor in Partially Transformed Rat Kidney Cells. Cancer Research, 46(11) 5842-5850.

Selected Presentations

  • A Zipper Network Model of Extracellular Matrix Failure Reveals a New Role for Proteoglycans - Biophysical Society Annual Meeting, - Boston, MA
  • In VSMCs, Beta-1 Integrin but not Syndecan-4 Gene Expression is Dependent on Matrix Stiffness - Biophysical Society Annual Meeting, - Boston, MA
  • Lysyl oxidase oxidizes basic fibroblast growth factor markedly reducing its mitogenic potential
  • Computational analysis of heparan sulfate-fibronectin binding: Impact on VEGF deposition in the extracellular matrix - American Chemical Society Annual Meeting, 2013 - New Orleans, LA
  • Corneal epithelial-neuronal signaling is compromised under environmental stress - ARVO Annual Meeting, 2013 - Seattle, WA
  • Control of VEGF-endothelial response by ECM stiffness - Experimental Biology Annual Meeting, 2013 - Boston, MA
  • Dynamic regulation of VEGF signaling by co-receptors and the extracellular matrix - Experimental Biology Annual Meeting, 2013 - Boston, MA
  • Hypoxia-induced heparan sulfate primes the extracellular matrix for cell recruitment by facilitating VEGF-fibronectin interactions. - Experimental Biology Annual Meeting, 2013 - Boston, MA
  • Computational Analysis of Heparan Sulfate Fibronectin Binding: Impact on VEGF Deposition in the Extracellular Matrix - ACS Symposium on Heparin: Challenges and Opportunities for a Biological Drug, April 2013 - New Orleans, LA
  • ÒVEGF, Endothelial Cells, and Angiogenesis: Activities, Assays, and IdeasÓ, April 2013 - Cambridge, MA
  • ÒComputational Analysis of Heparan Sulfate-Protein Binding SpecificityÓ - Boston Glycobiology Discussion Group, January 2013 - Newton, MA
  • Growth Factor-Extracellular Matrix Dynamics, January 2013 - Boston, MA
  • Extracellular Communication: Growth Factor-Extracellular Matrix Dynamics, December 2012 - Lowell, MA
  • Evaluating Fibronectin Conformation based on Biochemical and Mechanical Cues - American Society for Matrix Biology Annual Meeting, 2012 - San Diego, CA
  • Corneal epithelial-neuronal signaling is compromised under environmental stress, 2012 - Ft. Lauderdale, FL
  • Computational Analysis of Heparan Sulfate-Protein Binding Specificity - Gordon Research Conference on Proteoglycans, July 2012 - Andover, NH
  • Extracellular Matrix-Cell and Cell-Cell Interactions Control Smooth Muscle Cell Response to Substrate StiffnessÓ - FASEB Ressearch Conference on Smooth Muscle, June 2012 - Snowmass, CO
  • How the Extracellular Matrix Controls VEGF - Eyes to the Brain Seminar Series, April 2012 - Boston, MA
  • ÒWhat Does a Scientist Do?Ó, April 2012 - Bedford, MA
  • - 7th International Conference on Proteoglycans, 2011 - Sydney, Australia
  • Substrate stiffness and cell_cell interactions regulate vascular smooth muscle cell behavior in an ECM_dependent manner - Biomedical Engineering Society Annual Meeting, 2011 - Hartford, CT
  • Variable Stretch Increases mRNA Expression of Collagen in Lung Fibroblasts in a 3D Gelfoam Matrix - Biomedical Engineering Society Annual Meeting, 2011 - Hartford, CT
  • Vascular smooth muscle cell behavior is jointly regulated by substrate stiffness and cell-cell interactions - Biomedical Engineering Society Annual Meeting, 2011 - Hartford, CT
  • Control of VEGF binding to fibronectin by heparan sulfate and hypoxia, 2011
  • Variable Stretch Increases mRNA Expression of Collagen in Lung Fibroblasts in a 3D Gelfoam Matrix - European Biomedical Engineering Conference, 2011 - Budapest, Hungary
  • Extracellular matrix presentation modulates vascular smooth muscle cell mechanotransduction - NAVBO Workshops in Vascular Biology, 2011 - Hyannis, MA
  • Fibronectin, Perlecan and the VEGF/FGF2 Nexus in Vascular Biology - 7th International Conference on Proteoglycans, October 2011 - Sydney, Australia
  • Extracellular Communication: Growth Factor Dynamics in Tissue Injury and Repair, June 2011 - Boston, MA
  • Peeling the Heparan Sulfate Onion: Sequences, Domains, and Activities, April 2011 - Cambridge, MA
  • Biochemical and mechanical cues tune fibronectin conformation - Biannual Meeting of the American Society for Matrix Biology, 2010 - Charleston, SC
  • Intratumoral LOX-PP Injection or Slow Release Inhibits Growth of Pre-existing Murine Breast Cancer Xenografts - Biannual Meeting of the American Society for Matrix Biology, 2010 - Charleston, SC
  • Differential talin and vinculin expression during vascular smooth muscle cell mechanotransduction - Biomedical Engineering Society Annual Fall Meeting, 2010 - Austin, TX
  • Design of a Device for Mechanical Stimulation of Tissue-Engineered Constructs - Biomedical Engineering Society Annual Meeting, 2010 - Austin, TX
  • ChamP analysis of vascular heparan sulfate: Domain patterns, binding motifs, and much more - Gordon Research Conference on Proteoglycans, 2010 - Proctor Academy, Andover, NH
  • Control of Endothelial Progenitor Cell Differentiation on Fibronectin by Heparin/Heparan Sulfate - American Society of Matrix Biology Annual Meeting, October 2010 - Charleston. SC
  • Complex regulation of FGF binding and activity by heparan sulfate proteoglycans, August 2010 - Cambridge, MA
  • Sweet Matrix: How complex sugars modulate extracellular matrix function, April 2010 - Boston, MA
  • Matrix mechanics modulate expression of ECM receptors and contractile markers in VSMCs - Biomedical Engineering Society Annual Fall Meeting, 2009 - Pittsburg, PA
  • Neutrophil Elastase Regulates VEGF Activity via ECM Degradation and VEGF Fragment Generation - Biomedical Engineering Society Annual Fall Meeting, 2009 - Pittsburg, PA
  • Extracellular Communication: Growth Factor Dynamics in Tissue Injury and Repair, October 2009 - Cambridge, MA
  • The Many Faces of Heparin, August 2009 - Boston, MA
  • Extracellular Communication: Growth Factor Dynamics in Tissue Injury and Repair - Condensed Matter Seminar Series, March 2009 - Blacksburg, VA
  • Methods to investigate the effect of matrix mechanics and composition on cell signaling - 34th Annual Northeast Bioengineering Conference, 2008 - Brown University, Providence, RI
  • Cleavage of VEGF by neutrophil elastase generates a fragment with altered activity - Experimental Biology 2008, 2008 - San Diego, CA
  • Methods for investigating integrin-matrix interactions as a function of matrix mechanics and composition - Experimental Biology 2008, 2008 - San Diego, CA
  • Catalytic mechanisms involving heparan sulfate-fibronectin interactions - Gordon Research Conference on Proteoglycans, July 2008 - Andover, NH
  • A Catalytic Role for Heparin in the Extracellular Matrix: Regulation of VEGF-Fibronectin Interactions - Vascular Biology Seminar, February 2008 - Boston, MA
  • In silico modeling of interstitial lung mechanics and repair - Biomedical Engineering Society Annual Fall Meeting, 2007 - Los Angeles, CA
  • Mechanical and failure properties of collagen-based lung tissue constructs - Biomedical Engineering Society Annual Fall Meeting, 2007 - Los Angeles, CA
  • Regulation of pulmonary cell response to elastase injury by heparan sulfate proteoglycans - Biomedical Engineering Society Annual Fall Meeting, 2007 - Los Angeles, CA
  • Structural Catalysis by Heparin: The Fibronectin Paradigm - Science and Engineering Day, Boston University, 2007 - Boston
  • A New Class of Biological Catalysts: Linear Polysaccharides Catalyze a Conformational Change in Fibronectin in order to Coordinate Growth Factor Activity - The Protein Society 21st Annual Symposium, 2007 - Boston, MA
  • Direct and Indirect Regulation of Growth Factor Binding and Signaling by Proteoglycan Binding Competitors - AIChE meeting, November 2007 - Salt Lake City, UT
  • - 5th International Conference on Proteoglycans, September 2007 - Rio de Janeiro, Brazil
  • Catalysis of Conformational Changes in Fibronectin by Heparin and Heparan Sulfate - 5th International Conference on Proteoglycans, September 2007 - Rio de Janeiro, Brazil
  • Regulation of the Pulmonary Cell Response to Elastase Injury by Heparan Sulfate - Biomedical Engineering Society Meeting, September 2007 - Los Angeles, CA
  • Interactions of Vascular Endothelial Growth Factor with the Extracellular Matrix: Regulated Information Transfer, December 2006 - Boston, MA
  • Heparin mediates conformational changes on fibronectin exposing vascular endothelial growth factor binding sites - Experimental Biology Conference, 2006 - San Francisco, CA
  • Dynamics of cell regulation by proteoglycans - First Indo-US Frounteirs of Engineering Forum, March 2006 - Agra, India
  • Syndecan-4 mediates the assembly of focal adhesions and actin stress fibers through recruitment of alphaV beta3 in endothelial cells - American Heart Association Scientific Session 2005, 2005 - Dallas, TX
  • Linear polysaccharide chains as regulators of protein-protein interactions - Gordon conference on Proteins, 2005 - Plymouth, NH
  • Storage and Release of Growth Factors from the Extracellular Matrix: Nature's Controlled Release System, November 2005 - Cambridge, MA
  • Heparan sulfate modulation of VEGF-fibronectin interactions, December 2004 - Boston, MA
  • Complexities and consequences of growth factor interactions with heparan sulfate, October 2004 - Boston, MA
  • New Insights into the Inhibition of Human Neutrophil Elastase by Heparin and Heparan Sulfate. - The Gordon Research Conference on Proteolytic Enzymes & Their Inhibitors, July 2004 - Colby-Sawyer College, New London, NH
  • Control of growth factor storage, release and activity by the extracellular matrix, March 2004 - Palo Alto, CA
  • Regulation of FGF-2-induced ERK activation kinetics and localization in smooth muscle cells by extracellular matrix and cytoskeleton - American Society for Matrix Biology Meeting 2003, 2003 - San Diego
  • Heparan sulfates expressed in the distal lung are required for Fgf10 binding to the epithelium and for airway branching, 2003
  • Dynamics of growth factor regulation in cells and the extracellular matrix - Vascular Biology Seminar Series, May 2003 - Lebannon, NH
  • Control of heparin-binding growth factors by proteoglycans in smooth muscle cells - American Society for Cell Biology (ASCB) Annual Meeting, December 2002 - San Franscisco, CA
  • The nuclear localization of basic fibroblast growth factor is mediated by heparan sulfate proteoglycans through protein kinase C signaling - American Society for Cell Biology (ASCB) Annual Meeting, December 2002 - San Franscisco, CA
  • Complex Growth Factor/Receptor Interactions and Control of Cell Proliferation - 5th Annual Meeting of the GZG European Tissue Culture Society on Heterologous Cell Interactions & 3-D Cell Systems, 2002 - Regensburg, Germany
  • FGF Stimulation of MAP Kinase Signaling: Combining Experimental Analysis and Computational Modeling - American Institute of Chemical Engineers Annual Meeting, 2002
  • FGF-2 activation of ERK1/2 via heparan sulfate proteoglycans and lipid rafts - Gordon Conference on Proteoglycans, 2002 - Gordon Conference on Proteoglycans, Protor Academy, Andover, NH
  • Control of Growth Factor Action by Proteoglycans in the ECM and on Cell Surfaces - Gordon Research Conference on Signal Transduction by Engineered Extracellular Matrices, 2002 - Connecticut College in New London, CT
  • Extracellular pH modulates VEGF-heparan sulfate interactions and activity in vascular endothelial cells - International Congress on Eye Research, 2002 - Geneva, Switzerland
  • Proteoglycans and Thrombosis - AHA Scientific Sessions 2002, November 2002 - Chicago, IL
  • Changes in extracellular pH alter vascular endothelial growth factor-heparan sulfate interactions - The first meeting of The American Society for Matrix Biology, November 2002 - Houston, TX
  • Potential role of epidermal growth factor receptor in maintenance of elastogenic phenotype of lung fibroblasts - The first meeting of The American Society for Matrix Biology, November 2002 - Houston, TX
  • Extracellular pH modulates VEGF-heparan sulfate interactions and activity in vascular endothelial cells - XV International Congress of Eye Research, October 2002 - Geneva, Switzerland
  • New Insights into the Roles of Heparan Sulfate in Disease, September 2002 - Cambridge, MA
  • Complex Growth Factor/Receptor Interactions and Control of Cell Proliferation - 5th Annual Meeting of the GZG European Tissue Culture Society on Heterologous Cell Interactions & 3-D Cell Systems, June 2002 - Regensburg, Germany
  • Control of Growth Factor Action by Proteoglycans in the ECM and on Cell Surfaces - Gordon Research Conference on Signal Transduction by Engineered Extracellular Matrices, June 2002 - Connecticut College in New London, CT
  • Extracellular pH modulates vascular endothelial growth factor-heparan sulfate interactions - Experimental Biology 2002, April 2002 - New Orleans, LA
  • Mechanisms of FGF-2 induced MAP kinase activation - Experimental Biology 2002, April 2002 - New Orleans, LA
  • Proteoglycans regulate heparin-binding growth factors through lipid rafts - Experimental Biology 2002, April 2002 - New Orleans, LA
  • Proteoglycans regulate heparin-binding growth factor action in smooth muscle cells - Keystone Symposia: Biological Response to Extracellular Matrix, February 2002 - Banff, Alberta, Canada
  • Structure function relationships of glycosaminoglycans, February 2002 - Cambridge, MA
  • - 41st American Society for Cell Biology Annual Meeting, December 2001 - Washington, DC
  • Control of heparin-binding growth factors by proteoglycans in smooth muscle cells - American Society for Cell Biology (ASCB) Annual Meeting, December 2001 - Washington D.C.
  • Purification and characterization of biologically active corneal decorin, 2001
  • Heparan Sulfate Proteoglycans as Sites for Cellular Uptake of Extracellular Ligands, October 2001 - Alameda, CA
  • Modulation of Growth Factors in the Corneal Stroma by Heparan Sulfate Proteoglycans - The 2001 Biennial Cornea Research Conference, September 2001 - Boston, MA
  • Complex receptor/growth factor interactions governing endocytic trafficking and cell response - 28th International Symposium on Controlled Release of Bioactive Materials, June 2001 - San Diego, CA
  • Complex receptor/growth factor interactions governing endocytic trafficking and cell responses - 28th International Symposium on Controlled Release of Bioactive Materials, June 2001 - San Diego, CA
  • Proteoglycans regulate pulmonary fibroblast cellular response and extracellular matrix composition, March 2001 - Orlando, FL
  • Fibronectin directs nuclear localization of cell surface heparan sulfate proteoglycans., 2000
  • Heparan sulfate proteoglycans regulate extracellular matrix composition in pulmonary fibroblasts., 2000
  • Lysyl oxidase oxidizes basic fibroblast growth factor markedly reducing its mitogenic potential., 2000
  • Nuclear localization of heparan sulfate proteoglycans is regulated by fibronectin in corneal stromal fibroblasts., 2000
  • TGF-_ mediates the expression of TGF-_1 and FGF-2 in response to injury, 2000
  • Perivascular Endothelial Cell Transplantation - 3rd International Congress on Coronary Artery Disease _ From Prevention to Intervention, October 2000 - Lyon, France
  • Growth Factor-Proteoglycan Interactions: Regulation of Cell Function - Thomas Jefferson University, Cardeza Foundation for Hematologic Research Seminar, October 2000 - Philadelphia, PA
  • Regulation of Proteoglycan Expression: Impact on Growth Factor Binding and Activity - XIV International Congress of Eye Research, October 2000 - Sante Fe, NM
  • Tissue Engineered Endothelial Cell Transplantation - XIth International Vascular Biology Meeting, September 2000 - Geneva, Switzerland
  • Growth Factor Interactions with Cells - Gordon Research Conference on The Science of Adhesion, August 2000 - Tilton, NH
  • Regulation of Growth Factor Transport and Processing by Heparan Sulfate Proteoglycans - Gordon Research Conference on Proteoglycans, July 2000 - Andover, NH
  • Heparan Sulfate and Growth Factors: A Complex System of Cellular Control, March 2000 - Boston, MA
  • Regulation of Vascular Smooth Muscle Cell Growth, March 2000 - Boston, MA
  • Control of Vascular Smooth Muscle Cell Proliferation: The Molecular Basis of Restenosis - SCVIR 25th Annual Scientific Meeting, March 2000 - San Diego, CA
  • Decorin and transforming growth factor-_1 influence proteoglycan synthesis and proliferation in corneal fibroblasts, 1999
  • Heparan sulfate proteoglycans regulate accumulation and elastase-mediated release of bFGF in pulmonary fibroblasts - FASEB Annual Meeting, 1999 - Washington, D.C.
  • Injury induces transforming growth factor-_1 in rabbit corneal fibroblasts, 1999
  • Regulation of syndecan-4 in an in vitro model of corneal stromal fibroblast wound healing, 1999
  • Transport of Angiogenic Factors through the Extracellular Matrix - Boston Angiogenesis Meeting, November 1999 - Boston, MA
  • Growth Factor Binding, Trafficking, and Activity: The Complexities of Fibroblast Growth Factor, October 1999 - Blacksburg, VA
  • Transport of Growth Factors through the Extracellular Matrix: Implications for Drug Delivery and Tissue Engineering, October 1999 - Blacksburg, VA
  • Cell density dependence of basic fibroblast growth factor binding and activity - 38th ASCB Annual Meeting, 1998 - San Fransico, CA
  • Heparan Sulfate Proteoglycans Regulate the Intracellular Localization and Processing of Heparin Binding Growth Factors - 38th ASCB Annual Meeting, 1998 - San Francisco, CA
  • Heparan Sulfate Proteoglycans Regulate the Intracellular Localization and Processing of Heparin Binding Growth Factors - 38th ASCB Annual Meeting, 1998 - San Francisco, CA
  • Cell density regulates proteoglycan expression and bFGF responsiveness in cornea stromal fibroblasts - ARVO Annual Meeting, 1998 - Ft. Lauderdale, FL
  • Regulation of decorin and perelcan synthesis by TGF-_ in cultured stromal fibroblasts - ARVO Annual Meeting, 1998 - Ft. Lauderdale, FL
  • Effect of elastase on bFGF receptor function in neonatal rat lung fibroblast - FASEB Annual Conference, 1998 - San Fransico, CA
  • Development of a new in vivo model of pulmonary emphysema - FASEB Annual Meeting, 1998 - San Fransico, CA
  • Heparan Sulfate Mediated Transport of Growth Factors through Basement Membranes - Gordon Research Conference on Basement Membranes, 1998 - New England College, Henniker, NH
  • A Quantitative Analysis of Heparan Sulfate Mediated Transport of Growth Factors through Extracellular Matrix - Gordon Research Conference on Proteogylcans, 1998 - Proctor Academy, Andover, NH
  • Growth Factor Interactions with the Extracellular Matrix, November 1998 - Baltimore, MD
  • Growth Factor Interactions with the Extracellular Matrix - Massachusetts Institute of Technology, Langer Lab Research Seminar Series, November 1998 - Cambridge, MA
  • Fundamentals of Molecular Biology, October 1998 - Washington DC
  • Growth Factor Transport through the Extracellular Matrix: Oh what a Wicked Web, October 1998 - Boston, MA
  • Heparan Sulfate Mediated Transport of Growth Factors through Basement Membranes - Gordon Research Conference on Basement Membranes, June 1998 - New England College, Henniker, NH
  • Growth Factor-Proteoglycan Dynamics: Impact on Biological Activity - Massachusetts Institute of Technology, Division of Toxicology Seminar Series, March 1998 - Cambridge, MA
  • Heparan sulfate proteoglycans control basic fibroblast growth factor trafficking - American Institute of Chemical Engineering Annual Meeting, 1997 - Los Angeles, CA
  • Heparin regulation of bFGF binding: Mathematical modeling to understand complex phenomena - American Institute of Chemical Engineering Annual Meeting, 1997 - Los Angeles, CA
  • Synthesis of proteoglycans in the presence of TGF-_ by cultured stromal fibroblasts - ARVO Annual Meeting, 1997
  • Transport properties of Descemet's membrane - ARVO Annual Meeting, 1997
  • Development of a new in vivo model of pulmonary emphysema - East Coast Connective Tissue Society Meeting, 1997 - Boston, MA
  • Potentiation and inhibition of bFGF binding by heparin: A model for regulation of cellular response - East Coast Connective Tissue Society Meeting, 1997 - Boston, MA
  • Synthesis of glycosaminoglycans by cultured corneal fibroblasts - East Coast Connective Tissue Society Meeting, 1997 - Boston, MA
  • Transport properties of basement membrane - East Coast Connective Tissue Society Meeting, 1997 - Boston, MA
  • Growth factor transport through basement membrane - The Whitaker Foundation Biomedical Engineering Research Conference, 1997 - Snow Bird, Salt Lake City, UT
  • Fibroblast Growth Factors - Gordon Research Conference on Biomaterials: Biocompatibility and Tissue Engineering, July 1997 - Holderness School, NH
  • Regulation of bFGF Binding, Trafficking and Activity by Heparan Sulfate Proteoglycans - Harvard Medical School, Seminars in Vascular Biology, February 1997
  • Control of Growth Factor Activity by Proteoglycans - Cornea/Ocular Surface Seminar, December 1996
  • Altered glycosaminoglycan synthesis in platelet poor serum - 36th American Society for Cell Biology Annual Meeting, 1996
  • An in vivo model for transforming growth factor-_1 induced corporal fibrosis: Implications for penile ischemia-associated fibrosis, 1996
  • Endothelial proteoglycans inhibit bFGF binding, mitogenesis and angiogenesis - ARVO Annual Meeting, 1996
  • In vitro synthesis of proteoglycans by stromal fibroblasts in the absence of platelet associated growth factors - ARVO Annual Meeting, 1996
  • Control of growth factor binding and activity by proteoglycans - Center for Biomedical Engineering/Industrial Liaison Program Workshop on MIT/Industry Interactions in Biomedical Engineering, 1996 - Cambridge, MA
  • bFGF-Heparan Sulfate Dynamics: A Role for Both Cell Surface and Extracellular Proteoglycans in Controlling bFGF Binding and Activity - Gordon Research Conference on Proteoglycans, 1996
  • Growth factor transport through basement membrane - The Whitaker Foundation Biomedical Engineering Research Conference, 1996
  • Molecular Biology in Cardiovascular Drug Delivery - The Second Annual International Conference on Local Cardiovascular Drug Delivery, October 1996
  • Control of bFGF Binding, Transport and Activity by Heparan Sulfate, September 1996 - Skillman, NJ
  • bFGF-Heparan Sulfate Dynamics: A Role for Both Cell Surface and Extracellular Proteoglycans in Controlling bFGF Binding and Activity - Gordon Research Conference on Proteoglycans, July 1996
  • Growth Factor-Proteoglycan Dynamics, March 1996 - Boston, MA
  • A comparison of glycosaminoglycans and TGF-_ during corneal wound healing in culture and in vivo - 19th Cornea Research Conference, 1995 - Boston, MA
  • Quantitative analysis of basic fibroblast growth factor binding to endothelial cell-derived heparan sulfate proteoglycans - AIChe Annual Meeting, 1995
  • A quantitative in vivo assay for interleukin 1_ induced corneal inflammation - ARVO Annual Meeting, 1995
  • Synthesis of keratan sulfate by cultured keratocytes - ARVO Annual Meeting, 1995
  • FGF binds its receptors, is internalized and stimulates DNA synthesis in the absence of heparan sulfate - ASCB Annual Meeting, 1995 - Washington, D.C.
  • FGF binds its receptors, is internalized and stimulates DNA synthesis in the absence of heparan sulfate - Gordon Research Conference on Angiogenesis and Microcirculation, 1995
  • Tissue engineered endothelial-cell implants and proliferative vascular-disease, 1995
  • Tissue Engineered Endothelial Cell Implants and Proliferative Vascular Disease - American Heart Association, 68th Scientific Sessions, November 1995 - Anaheim, CA
  • Efficiencies of local vascular delivery of heparin and heparin-binding growth factors differ depending on compound, artery, and site of administration - American Heart Association Annual Meeting, 1994
  • A comparison of the biosynthesis of proteoglycans during corneal wound healing in culture and in vivo. - ARVO Annual Meeting, 1994
  • Inhibition of bFGF stimulated capillary endothelial cell proliferation by aqueous humor and corneal endothelial cell conditioned medium. - ARVO Annual Meeting, 1994
  • Synthesis of proteoglycans and TGF-_1 expression in response to corneal injury in vitro and in vivo - ASCB Annual Meeting, 1994 - San Francisco, CA
  • Endothelial cell-derived heparan sulfates modulate basic fibroblast growth factor binding to vascular smooth muscle cells - Tissue Engineering Session, 1994 AIChe Annual Meeting, 1994
  • Aqueous humor contains and corneal endothelial cells secrete proteoglycans that inhibit bFGF binding and activity on capillary endothelial cells - Vascular Cell Biology Gordon Conference, 1994
  • Control of Vascular Smooth Muscle Cell Proliferation - Boston University School of Medicine, Graduate Student Invitational Seminar Series, January 1994 - Boston, MA
  • The continuous release of bFGF to enhance the delay phenomenon in skin flaps: An experimental study - Plastic Surgery Research Council, 1993
  • The effect of fibroblast growth factor on wound angiogenesis and epithelialization in the hairless mouse ear wound model. - Plastic Surgery Research Council, 1993
  • Growth Factors and Proteoglycans in the Control of Vascular Cell Growth, March 1993 - Boston, MA
  • Controlled Delivery of Polypeptide Growth Factors - Gordon Research Conference on Drug Carriers in Biology and Medicine, 1992
  • Controlled Release of Basic Fibroblast Growth Factor and Cell Growth Control, July 1992 - Cambridge, MA
  • Controlled Delivery of Polypeptide Growth Factors - Gordon Research Conference on Drug Carriers in Biology and Medicine, July 1992
  • Controlled Release of Basic Fibroblast Growth Factor and Cell Growth Control, April 1992 - Providence, RI
  • Control of Cell Proliferation by Growth Factors and the Extracellular Matrix - Georgia Institute of Technology, Department of Biology, March 1992 - Atlanta, GA
  • Growth Factors, the Extracellular Matrix and Tissue Engineering, March 1992 - Baltimore, MD
  • Regulation of bFGF by TGF-beta and Proteoglycans, February 1992 - Charlestown, MA
  • Controlled Release of Fibroblast Growth Factor - Materials Research Society Fall Meeting, Symposium T: Tissue-Inducing Biomaterials, December 1991
  • Basic fibroblast growth factor enhances the coupling of intimal hyperplasia and proliferation of vasa vasorum in injured rat arteries. - American Heart Association Annual Meeting, 1991
  • Differential binding of basic fibroblast growth factor to extracellular matrix and cell surface receptors results from a difference in off rates not on rates. - FASEB Annual Meeting, 1991
  • Polymer Based Delivery Systems for Growth Factors. - Gordon Research Conference on Biocompatibility and Biomaterials, 1991
  • Controlled Release of Fibroblast Growth Factor - Materials Research Society Fall Meeting, Symposium T: Tissue-Inducing Biomaterials, 1991
  • TGF_1 stimulates the production of bFGF-binding heparan sulfate proteoglycans and potentiates bFGF-stimulated DNA synthesis in Balb/c3T3 cells. - New York Academy of Sciences Meeting on the Fibroblast Growth Factor Family, 1991
  • Polymer Based FGF Delivery Systems and Smooth Muscle Cell Proliferation, November 1991 - Providence, RI
  • Polymer Based Delivery Systems for Growth Factors. - Gordon Research Conference on Biocompatibility and Biomaterials, July 1991
  • Inhibition of NRK fibroblast growth by TGF-_1 is mediated by collagen: Loss of alpha 2 (I) collagen expression and growth inhibition by TGF-_1 in transformed NRK cells. - FASEB Conference on Metastasis, 1990
  • Growth Regulation by TGF-beta., March 1990 - Boston, MA
  • Growth Regulation by TGF-beta - Tufts University School of Medicine, Department of Anatomy and Cellular Biology, March 1990 - Boston, MA
  • Inhibition of normal rat kidney cell growth by transforming growth factor-_ is mediated by collagen - Gordon Conference on Molecular and Genetic Basis for Cell Proliferation, 1989
  • Inhibition of normal rat kidney cell growth by transforming growth factor-_ is mediated by collagen - New York Academy of Sciences Meeting on Transforming Growth Factor-_s: Chemistry, Biology and Therapeutics, 1989
  • Growth stimulation, altered regulation of epidermal growth factor receptors, and autocrine transformation of spontaneously transformed normal rat kidney cells by transforming growth factor-_. - FASEB Conference on Receptors, 1988
  • Growth stimulation, altered regulation of epidermal growth factor receptors, and autocrine transformation of spontaneously transformed normal rat kidney cells by transforming growth factor - Gordon Conference on Cancer, 1988

Selected Intellectual Property

  • - Nugent, M.A."Inhibition of Vascular Occlusion Following Vascular Intervention," European Patent EP0850073
  • - Nugent, M.A."Inhibition of occlusion of blood vessel after intervening blood vessel," Japanese Patent JP2005254000
  • - Nugent, M.A."Method to inhibit histone acetyltransferase using glycosaminoglycans," U.S.S.N. 60/584,358
  • Patent - Nugent, M.A."Method for stimulating angiogenesis and wound healing," U.S.S.N. 60/461,974
  • - Nugent, M.A."Inhibition of vascular smooth muscle cell proliferation with implanted matrix containing vascular endothelial cells," United States Patent Number 5,766,584 United States
  • Patent - Nugent, M.A."Method for inhibiting angiogenesis using heparinase," Patent Number 5,567,417; International patent no. PCT/US94/13343

Selected Contracts, Fellowships, Grants and Sponsored Research

  • Improve musculoskeletal Health especially among the elderly (2018), Grant - IDEA Inter-Disciplinary Exchange & Advancement Initiative
    Lewis, E.S., Gage, M., Nugent, M.A., Mangano, K., Konow, N., Scribner-Maclean, M.