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Jin Xu

Jin Xu
Jin XuAssociate Professor
  • CollegeCollege of Sciences
  • DepartmentChemistry
  • Phone(978) 934-4000
  • EmailJin_Xu@uml.edu

Research Interests

Biopharmaceutical development; Biochemical, biophysical, and mass spectrometry characterization of protein structure; protein aggregation mechanisms; neurodegenerative and cardiovascular diseases related to protein aggregation.

Education

  • Ph D: Biochemistry, (2000), University of North Texas - Denton, Texas
    Dissertation/Thesis Title: Conformational Studies of Myosin and Actin with Calibrated Resonance Energy Transfer
  • BS: Biochemistry, (1989), East China University of Science and Technology - Shanghai, China

Publications

  • Harrison, T., Oakley, A., Thota, S., Carter, T., Xu, J., Sandman, D.J. (2015) "Approaches to new solid state reactions," Abstracts of Papers, 250th ACS National Meeting & Exposition, Boston, MA, United States, August 16-20, 2015 pp. ORGN-185
  • Strand, J., Huang, C., Xu, J. (2013) "Characterization of high molecular weight aggregates from a CHO-expressed ECD receptor-Fc fusion protein," Journal of pharmaceutical sciences 102: pp. 441-453
  • Joglekar, M., Ware, J., Xu, J., Fitzgerald, M.f., Gartner, T. (2013) "Platelets, glycoprotein Ib-IX, and von Willebrand factor are required for FeCl3-induced occlusive thrombus formation in the inferior vena cava of mice," Platelets 24:3 pp. 205-212
  • Bhoskar, P., Belongia, B., Smith, R., Yoon, S., Carter, T., Xu, J. (2013) "Free light chain content in culture media reflects recombinant monoclonal antibody productivity and quality," Biotechnology Progress 29:5 pp. 1131-1139
  • Schirmer, E., Golden, K., Xu, J., Milling, J., Murillo, A., Lowden, P., Mulagapati, S., Hou, J., Kovalchin, J., Masci, A., Collins, K., Zarbis-Papastoitsis, G. (2013) "Reduction of product-related species during the fermentation and purification of a recombinant IL-1 receptor antagonist at the laboratory and pilot scale," Biotechnology Journal 8:8 pp. 946-956
  • Strand, J., Huang, C., Xu, J. (2013) "Characterization of Fc-fusion protein aggregates derived from extracellular domain disulfide bond rearrangements," Journal of Pharmaceutical Sciences 102:2 pp. 441-453
  • Wang, P., Liu, M., Sun, H., Xu, J. (2012) "A QCM-based Lab-On-A-Chip device for real time characterzation of shear-Induced platelets adhesion and aggregation. Proceedings of the 10th International Conference on Nanochannels,," Microchannels and Minichannels.
  • Lee, H.W., Christie, A., Xu, J., Yoon, S. (2012) "Data fusion-based assessment of raw materials in mammalian cell culture," Biotechnology and bioengineering
  • Joglekar, M., Ware, J., Xu, J., Fitzgerald, M.E., Gartner, K.T. (2012) "glycoprotein Ib-IX, and von Willebrand factor are required for FeCl3-induced occlusive thrombus formation in the inferior vena cava of mice," Platelets
  • Belongia, B.M., Smith, R.M., McGlothlen, M.J., Xu, J., Bhoskar, P. (2011) "Process and product quality improvements of a challenging monoclonal antibody," Abstracts of Papers of the American Chemical Society 241:
  • Wang, Q., Shorten, D., Xu, X., Shaw, G., Schaub, R., Shea, C., Brooks, J., Sako, D., Wiswall, E., Xu, J., Szklut, P., Patel, V. (2006) "Effect of von Willebrand Factor on the Pharmacokinetics of Recombinant Human Platelet Glycoprotein Ib -Immunoglobulin G1 Fusion Protein," Pharmaceutical research 23: pp. 1743-1749
  • Wang, X., Cooper, K.L., Wang, A., Xu, J., Wang, Z., Zhang, Y., Tu, Z. (2006) "Label-free DNA sequence detection using oligonucleotide functionalized optical fiber," Applied Physics Letters 89:16
  • Gundapaneni, D., Xu, J., Root, D. (2005) "High Flexibility of the Actomyosin Crossbridge Resides in Skeletal Muscle Myosin Subfragment-2 as Demonstrated by a New Single Molecule Assay," Journal of structural biology 149: pp. 117-126
  • Qin, J., Zhou, Y., Xu, J., Chen, H. (2005) "Studying the structure of terrorist networks: A web structural mining approach," 2: pp. 523-530
  • Huang, J.C., Xu, J. (2003) "Thermal degradation of polypropylene in a capillary rheometer," International Journal of Polymeric Materials 52:3 pp. 203-209
  • Root, D., Stewart, S., Xu, J. (2002) "Dynamic Docking of Myosin and Actin Observed with Resonance Energy Transfer," Biochemistry 41: pp. 1786-1794
  • Xu, J., Root, D. (2000) "Conformational Selection during Weak Binding at the Actin and Myosin Interface," Biophysical journal 79: pp. 1498-1510
  • Root, D., Shangguan, X., Xu, J., McAllister, M. (1999) "Determination of Fluorescent Probe Orientations on Biomolecules by Conformational Searching: Algorithm Testing and Applications to the Atomic Model of Myosin," Journal of structural biology 127: pp. 22-34
  • Xu, J., Root, D. (1998) "Domain Motion between the Regulatory Light Chain and the Nucleotide Site in Skeletal Myosin," Journal of structural biology 123: pp. 150-161
  • Xu, J., Gross, R.A., Kaplan, D.L., Swift, G. (1996) "Chemoenzymatic synthesis and study of poly( -methyl- -propiolactone) stereocopolymers," Macromolecules 29:13 pp. 4582-4590
  • Xu, J., McCarthy, S.P., Gross, R.A., Kaplan, D.L. (1996) "Chitosan film acylation and effects on biodegradability," Macromolecules 29:10 pp. 3436-3440
  • Svirkin, Y.Y., Xu, J., Gross, R.A., Kaplan, D.L., Swift, G. (1996) "Enzyme-catalyzed stereoelective ring-opening polymerization of -methyl- -propiolactone," Macromolecules 29:13 pp. 4591-4597
  • Gross, R.A., Xu, J., Svirkin, Y.Y., Henderson, L.A., Bisht, K.S., Kaplan, D.L., Swift, G. (1996) "Enzymes in organic media as catalysts for polyester synthesis," Abstracts of Papers of the American Chemical Society 211:
  • Xu, J., McCarthy, S.P., Gross, R.A. (1996) "Racemic -methyl- -propiolactone polymerization by organometallic catalyst systems," Macromolecules 29:13 pp. 4565-4571
  • Jedlinski, Z., Kowalczuk, M., Kurcok, P., Adamus, G., Matuszowicz, A., Sikorska, W., Gross, R.A., Xu, J., Lenz, R.W. (1996) "Stereochemical control in the anionic polymerization of -butyrolactone initiated with alkali-metal alkorides," Macromolecules 29:11 pp. 3773-3777
  • Xu, J. (1995) "Isolation of Factor VIII from Human Plasma by Affinity Absorption," Chinese Journal of Blood transfusion 8: pp. 6-12
  • Xu, J., Lu, S. (1993) "Purification of FVIII Complex," Chinese Journal of Blood transfusion 6: pp. 9-13
  • Xu, J., Yang, G., Gu, Q. (1991) "Immobilization of Penicillin Acylase on Chitosan Matrix," Chinese Journal of Antibiotics 15: pp. 338-345

Presentations

  • Characterization of Highly Heterogeneous Proteins: Fc-Fusion Proteins - BioProcessing International Ð Europe 2012, April 2012 - Prague, Czech Republic
  • An intrinsic protein promotes amyloid beta aggregation - Biopharmaceutical Summit 2012, March 2012 - Lowell, MA
  • Characterization of high molecular weight aggregates from a CHO-expressed ECD receptor-Fc fusion protein - Biopharmaceutical Summit 2012, March 2012 - Lowell, MA
  • Effect of excess light chain expression on antibody productivity and structural integrity - Biopharmaceutical Summit 2012, March 2012 - Lowell, MA
  • Technology comparison for measuring metabolic components in mammalian cell culture processes - Biopharmaceutical Summit 2012, March 2012 - Lowell, MA
  • The characterization of oligosaccharide structures of a fusion glycoprotein - Biopharmaceutical Summit 2012, March 2012 - Lowell, MA
  • The role of disulfide bond formation in GPIb-Fc aggregation - Biopharmaceutical Summit 2012, March 2012 - Lowell, MA
  • Characterization of high molecular weight aggregates from a CHO-expressed ECD receptor-Fc fusion protein, January 2012 - San Francisco, CA
  • Process and Product Quality Improvements of a Challenging Monoclonal Antibody - 241st ACS National Meeting, March 2011 - Anaheim, CA
  • Characterization of a complex biotherapeutic throughout a process development cycle - Preclinical Scale Bioprocessing, November 2010 - Boston, MA
  • Evaluation of glycosylation throughout process changes in the development of a biotherapeutics - International BioAnalytical Congress: Post-Translational Modifications, September 2010 - Brussels, Belgium
  • The role of disulfide bond shuffling in GPIb-Fc aggregation - AAPS National Biotechnology Conference, May 2010 - San Francisco, CA
  • Characterization of high molecular weight aggregates from a CHO-expressed ECD receptor-Fc fusion protein - National Biotechnology Conference, May 2010 - San Francisco, CA
  • Characterization of a complex highly glycosylated protein by cIEF and correlations to protein heterogeneity, January 2010 - Washington, DC
  • - US/Ireland Emerging Technologies Conference, October 2009
  • Thiol Chemistry and protein aggregation - UMass Dartmouth Chemistry Seminar, September 2009 - Framingham, MA
  • Protein and analytical sciences at Massachusetts BioManufacturing Center - Waters Collaborators Annual Conference, November 2008 - Framingham, MA
  • GPIbalpha intermolecular disulfide bond shuffling and its potential physiological role in platelet activation and aggregation - Gordon Research Conference (Hemostasis), June 2008 - Waterville Valley, NH
  • Tackling protein aggregation, March 2008 - Cambridge, MA
  • Co-precipitation of DNA and mAb products in cell culture media, June 2007 - Cambridge, MA
  • Disulfide Bond Shuffling in GPG-290 Aggregation - Gordon Research Conference (Thiol-Based Redox Regulation & Signaling), 2006
  • Characterizing the heterogeneity of GPG-290 glycosylation and sulfation, August 2006 - Oceanside, CA
  • From intermolecular hydrophobic interaction to disulfide bond shuffling: the molecular pathway of GPG-290 aggregation, August 2006 - South San Francisco, CA
  • Changes in antibody weak cation exchange chromatographic profile: chemical or structural cause, July 2006 - Cambridge, MA
  • N-linked Glycosylation and its role in GPG-290 structural stability and ligand binding, July 2006 - Longwood, CO
  • Light chain over-expression and its impact on antibody productivity and quality, May 2006 - Andover, MA
  • The Role of Disulfide Bond Shuffling in GPG-290 Aggregation - Gordon Research Conference (Protein), 2005
  • Structural and functional characterization of posttranslational modifications on protein therapeutics, September 2005 - Cambridge, MA
  • Unpaired Cys residue and disulfide bond shuffling in GPG-290 aggregation, March 2005 - Andover, MA
  • Enzymatic Removal of N-linked Glycans from GPG-290 and the Subsequent Impact on Protein Structural Stability and Ligand Binding - Gordon Research Conference (Hemostasis), 2004
  • The impact of valine mutations in the regulatory loop and tyrosine sulfation states at the C-terminal region on GPIbalpha ligand binding, March 2004 - Andover, MA
  • Protein structural characterization sets the foundation for analytical development, February 2003 - Andover, MA
  • Purity analysis for the collagen sponge as BMP-2 carrier, November 2001 - Andover, MA
  • Structural changes at the actomyosin interface - Gordon Research Conference (Motility), 1999
  • Development of purification process and analytical methods for high-purity coagulation factor VIII - Chinese Annual Hemostasis and Thrombosis Meeting, 1995 - Suzhou, China

Contracts, Fellowships, Grants and Sponsored Research

  • Structural Characterization and Analytical Development for an 11 Bio Product (2011), -
    Xu, J. (Principal)
  • De novo Sequencing of two mAbs (2014), -
    Xu, J. (Principal)
  • MU1141 Analytical Development and Structural Characterization (2014), -
    Xu, J. (Principal)
  • Analytical Development and Characterization of Prolanta from BioVectra (2010), -
    Xu, J. (Principal)
  • Mass Analysis of Dyax mAb Products and Associated Isoforms (2010), -
    Xu, J. (Principal)
  • Analytical Development and Characterization of Polymer Breakdown Products (2013), -
    Xu, J. (Principal)
  • Initial Characterization of INTICA Antibody Samples (2013), -
    Xu, J. (Principal)
  • Purification of Monoclonal Antibodies with High Capacity Membrane Absorbers (2012), -
    Xu, J. (Principal)
  • Structural Characterization of a Phase III Cancer Drug (2011), -
    Xu, J. (Principal)
  • Development of Generic Trastuzumab for the Treatment of Breast Cancer (2012), Contract - NATL CANCER INSTITUTE
    Xu, J. (Principal)
  • Product Characterization Method Development & Analytical Work (2010), -
    Xu, J. (Principal)
  • Primary Structural Characterization of STC Trastuzumab (2011), Contract - NATL CANCER INSTITUTE
    Xu, J. (Principal)
  • Sequence Analysis and Structural Characterization of a Yeast Protein by Peptide M (2010), -
    Xu, J. (Principal)
  • Quantitative Assessment of the Purity of a Glycoprotein Reagent (2011), - NIH-NATL INST OF ALLERGY & INFEC DISEASE
    Xu, J. (Principal)
  • Development of an SEC-MALLS Method for a Taligen Fusion Protein Product (2010), -
    Xu, J. (Principal)
  • Protein Characterization Package for investigatin Process-Related Heterogeneity (2009), -
    Xu, J. (Principal)
  • Application of the Corona Charged Aerosol Detector (CAD) in Antibody Analysis and (2008), -
    Xu, J. (Principal)
  • Evaluating Natrix Devices in mAb Purification (2010), -
    Xu, J. (Principal)
  • Analytical Development and Characterization of Asparaginase and Pegylated Asparag (2009), -
    Xu, J. (Principal)
  • Analysis of a Fab Product Using SEC, RPC and SDS-PAGE (2009), -
    Xu, J. (Principal)
  • RobotStudio: A Modular IDE-Based Holistic Approach to Teaching Language Engineeri (2008), Grant -
    Xu, J. (Principal)
  • Active Learning with TabletPC and Wiki: Using Mobile Computing and Wiki-based Web (2007), Grant -
    Xu, J. (Principal)
  • Analytical Development and Aggregation Characterization for a Fusion Protein Prod (2008), Grant -
    Xu, J. (Principal)