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Jeffrey R. Moore

Moore
Jeffrey R. MooreAssociate Professor

Research Interests

The study of molecular mechanics and cellular motility. Cell motility, regulation of cell shape and the mechanism and regulation of muscle contraction.

Our lab’s main interest is the study of molecular mechanics and cellular motility. We employ single molecule fluorescence techniques and laser trapping assays to study: the mechanism and regulation muscle contraction, cell motility, and the regulation of cell shape. Our lab’s investigations of cardiac muscle contraction focus on the effects of cardiomyopathy-causing amino acid substitutions on myosin’s mechanical and enzymatic properties and tropomyosin’s ability to regulate muscle contraction via the calcium/troponin system. We are also investigating actin filament network mechanics and the regulation of cytoskeletal dynamics.

Education

  • Other: Molecular Physiology & Biophysics, (2003), University of Vermont - Vermont
  • Ph D: Cell & Molecular Biology, (1999), University of Vermont - Vermont
  • MS: Biological Sciences, (1993), UMass Lowell - Massachusetts
  • BS: Biological Sciences, (1991), University of Lowell (UL) - MA

Publications

  • Achal, M., Trujillo, A.S., Melkani, G.C., Farman, G.P., Ocorr, K., Viswanathan, M.C., Kaushik, G., Newhard, C.S., Glasheen, B.M., Melkani, A., Suggs, J.A., Moore, J.R., Swank, D.M., Bodmer, R., Cammarato, A., Bernstein, S.I. (2016) "A Restrictive Cardiomyopathy Mutation in an Invariant Proline at the Myosin Head/Rod Junction Enhances Head Flexibility and Function, Yielding Muscle Defects in Drosophila.," Journal Of Molecular Biology 428:11 pp. 2446 - 2461
  • Sewanan, L.R., Moore, J.R., Lehman, W., Campbell, S.G. (2016) "Predicting Effects of Tropomyosin Mutations on Cardiac Muscle Contraction through Myofilament Modeling.," Frontiers In Physiology 7: pp. 473
  • Moore, J.R., Campbell, S.G., Lehman, W. (2016) "Structural determinants of muscle thin filament cooperativity.," Archives Of Biochemistry And Biophysics 594: pp. 8 - 17
  • Fischer, S., Rynkiewicz, M.J., Moore, J.R., Lehman, W. (2016) "Tropomyosin diffusion over actin subunits facilitates thin filament assembly.," Structural Dynamics (Melville, N.Y.) 3:1 pp. 012002
  • Karabina, A., Kazmierczak, K., Szczesna-Cordary, D., Moore, J.R. (2015) "Myosin regulatory light chain phosphorylation enhances cardiac ß-myosin in vitro motility under load," Archives of Biochemistry and Biophysics 580: pp. 14-21
  • Schmidt, W.M., Lehman, W., Moore, J.R. (2015) "Direct observation of tropomyosin binding to actin filaments," Cytoskeleton 72:6 pp. 292-303
  • Lehman, W., Medlock, G., Li, X.E., Suphamungmee, W., Tu, A., Schmidtmann, A., Ujfalusi, Z., Fischer, S., Moore, J.R., Geeves, M.A., Regnier, M. (2015) "Phosphorylation of Ser283 enhances the stiffness of the tropomyosin head-to-tail overlap domain," Archives of Biochemistry and Biophysics 571: pp. 10-15
  • Egan, P., Moore, J.R., Schunn, C., Cagan, J., LeDuc, P. (2015) "Emergent systems energy laws for predicting myosin ensemble processivity," PLoS Computational Biology 11:4 pp. e1004177
  • Farman, G.P., Muthu, P., Kazmierczak, K., Szczesna-Cordary, D., Moore, J.R. (2014) "Impact of familial hypertrophic cardiomyopathy-linked mutations in the NHsub 2 terminus of the RLC on [Beta]-myosin cross-bridge mechanics," Journal of applied physiology 117:12 pp. 1471
  • Orzechowski, M., Fischer, S., Moore, J.R., Lehman, W., Farman, G.P. (2014) "Energy landscapes reveal the myopathic effects of tropomyosin mutations," Archives of Biochemistry and Biophysics 564: pp. 89-99
  • Muthu, P., Liang, J., Schmidt, W., Moore, J.R., Szczesna-Cordary, D. (2014) "In vitro rescue study of a malignant familial hypertrophic cardiomyopathy phenotype by pseudo-phosphorylation of myosin regulatory light chain," Archives of Biochemistry and Biophysics 552-553: pp. 29-39
  • Jensen, M., Morris, E., Gallant, C., Morgan, K., Weitz, D., Moore, J. (2014) "Mechanism of calponin stabilization of crosslinked actin networks," Biophysical Journal 106: pp. 793-800
  • Orzechowski, M., Moore, J.R., Fischer, S., Lehman, W. (2014) "Tropomyosin movement on F-actin during muscle activation explained by energy landscapes," Archives of Biochemistry and Biophysics 545: pp. 63-68
  • Huang, W., Liang, J., Kazmierczak, K., Muthu, P., Duggul, D., Farman, G.P., Sorenson, L., Pozios, I., Abraham, T.P., Moore, J.R., Borejdo, J., Szczesna-Cordary, D. (2014) "Hypertrophic cardiomyopathy associated Lys104Glu mutation in the myosin regulatory light chain causes diastolic disturbance in mice," Journal of Molecular and Cellular Cardiology 74: pp. 318-329
  • Guo, M., Ehrlicher, A.J., Jensen, M.H., Renz, M., Moore, J.R., Goldman, R.D., Lippincott-Schwartz, J., Mackintosh, F.C., Weitz, D.A. (2014) "Probing the Stochastic, Motor-Driven Properties of the Cytoplasm Using Force Spectrum Microscopy," Cell 158:4 pp. 822-832
  • Saphirstein, R., Gao, Y., Jensen, M., Gallant, C., Vetterkind, S., Moore, J.R., Morgan, K. (2013) "The focal adhesion: a regulated component of aortic stiffness," PLoS One 23:8 pp. 4 e62461
  • Guo, M., Ehrlicher, A., Mahammad, S., Fabich, H., Jensen, M., Moore, J.R., Fredberg, J., Goldman, R., Weitz, D. (2013) "The role of vimentin intermediate filaments in cortical and cytoplasmic mechanics," Biophysical Journal 105:7 pp. 1562-1568

Presentations

  • - Department of Medicine Cardiovascular Institute, - Boston University School of Medicine-
  • Cardiac Myosin Force Production: Fifty Ways to Love Your Lever - Department of Biological Sciences, 2014 - UMass Lowell
  • Myosin Mutations and Phosphorylation in Cardiac Disease - Department of Biological Sciences, 2014 - UMass Lowell
  • The Role of Myosin Phosphorylation in Cardiomyopathy Mutation Phenotypes - Squishy Physics Seminar, 2012 - Harvard University, Physics Department- Cambridge, MA
  • Myosin Lever Arm Mutations and Cardiomyopathy - Center for Muscle Biology, 2011 - University of Kentucky- Lexington, KY
  • Familial Hypertrophic Cardiomyopathy Alters Strain Dependent Kinetics - Muscle Palooza- Renssellaer Polytechnic Institute, 2010 - Troy, NY
  • The Myosin Lever Arm and Strain Dependent Biochemistry - Biology Department Renssellaer Polytechnic Institute, 2009 - Troy, NY
  • The Myosin Lever Arm and Cardiomyopathy - Department of Medicine- Wittaker Cardiovascular Institute, 2009 - Boston University- Boston, MA
  • The Myosin Lever Arm and Cardiomyopathy - Department of Mechanical Engineering, 2008 - Boston University- Boston, MA
  • Molecular Mechanics of Myosin and Actin - Department of Molecular and Cellular Pharmacology, 2007 - University of Miami- Miami, FL
  • Single Molecule Measurements of the Myosin II and Myosin V Working Stroke - Single Molecule Discussions, 2007 - Harvard University- Cambridge, MA
  • The Role of the Myosin Neck Region - Department of Medicine Cardiovascular Institute, 2005 - Boston University School of Medicine- Boston, MA
  • The Role of the Myosin Neck Region - Boston Biomedical Research Institute, 2004 - Watertown, MA
  • Structural and Functional Adaptations of Myosin: A Tale of Two Motors - Department of Veterinary and Comparative Anatomy- Pharmacology and Physiology, 2004 - Washington State- Pullman, WA
  • Structural and Functional Adaptations of Myosin: A Tale of Two Motors - Cardiovascular Institute, 2003 - Boston University School of Medicine- Boston, MA
  • The Role of the Myosin Neck Region - Department of Molecular and Cellular Biology, 2003 - Boston University Goldman School of Dental Medicine- Boston, MA
  • Motility Subgroup (Selected Presenter) - Biophysical Society Annual Meeting, 2002 - Boston, MA
  • Structural and Function Adaptations of Myosin: A Tale of Two Motors - Meakins Christie Laboratories, 2002 - Mcguill University- Montreal, Canada
  • Molecular Mechanics of Expressed Myosin II and Myosin V - 18th Annual Spring Symposium- New England Society for Microscopy, 2001 - Woods Hole Oceanographic Institute- Woods Hole, MA
  • Molecular Mechanics of Expressed Myosin II and Myosin V - Fifth International Muscle Energetics Conference, 2001 - Burlington, VT
  • Structure/Function Studies of Myosin II and Myosin V - Single Molecule Biophysics Symposium, 2001 - Aspen Center for Physics- Aspen, CO