Illumina NextSeq500 Sequencing
Illumina Inc. is arguably the global leader in next-generation sequencing solutions in breadth of applications, in quality of data and in reliability of instrumentation. At the UMass Lowell Biomolecular Characterization Laboratory we have a dedicated sample preparation and NGS expert to help you achieve your project goals on the NextSeq500. We are happy to offer free up-front consultation about clients’ planned experiments. Below is a simplified workflow outlining the process of completing an NGS project at the Biomolecular Characterization Laboratory.
- Consult the core about proposed experiment and complete experiment design.
- Extract and QC nucleic acid template (RNA or DNA) for Illumina library preparation chemistry.
- Convert your nucleic acid material into Illumina libraries at the core.
- QC, normalize, and load your libraries into the NextSeq500 and sequence.
- Data is delivered as demultiplexed FASTQ files to the Illumina BaseSpace cloud, or by FTP to the UMass Lowell server.
The Illumina NextSeq500 is capable of producing single-end sequences or more commonly, paired-end sequences of various lengths up to 300 bp single-end, or 150 bp paired-end.
Currently, the turn-around-time for samples is about 7 days from nucleic acid delivery through raw data generation.
The Biomolecular Characterization Laboratory is unique in that we provide three modes of usage. Clients can elect to be trained by the expert staff on sample QC, library preparation, and sequencer setup and operation (samples provided by client). Once a client is sufficiently trained (generally 2-3 training sessions, the client will qualify for a reduced self-use rate.) Alternatively, the client can elect to use the core for “service” and have the expert staff complete sample QC through sequencing for the client.
Financial and project consultation is free. If you would like to discuss your project with core staff and/or with Illumina, or if you would like a cost estimate, you can setup an appointment by emailing: Jack_Lepine@uml.edu.
If you would like a letter of support from the Biomolecular Characterization Laboratory, please contact the director of UMass Lowell Core Research Facilities, Teri Hamelin, at: Theresa_Hamelin@uml.edu.