(Located in Olney G03)
The ProteOn XPR36 protein interaction system is an SPR biosensor that provides real-time data on affinity, specificity, and interaction kinetics of protein interactions. The interaction analysis can look at interactions between DNA-DNA, DNA-protein, lipid-protein, and hydrid systems of biomolecules and non-biological surfaces. SPR can be used for antibody characterization, large and small molecule drug development, protein interface analysis, and protein complex and cascade analysis.
One interactant is immobilized on the sensor surface (ligand), while the other is free in solution and passed over the surface (analyte). The ProteOn XPR36 offers the ability to monitor up to 36 different interactions in real time, by processing, in parallel, 6 different ligands with 6 different analytes.
- Analyze up to 36 different protein interactions in a single run, on a single chip
- Identify the binding of two or more interactants to each other
- Determine the affinity of the interactions
- Measure the association and dissociation rates
- Screen multiple panels of analytes
- No radiochemical or fluorescent labels required
For the analysis of biomolecular interactions, the SPR uses ProteOn Manager software. ProteOn Manager offers easy to learn workflow, manual or automated processing, and wizards to simplify kinetic, equilibrium, and concentration analysis. The software generates reports of results including sensorgrams and graphs.
Users will need to provide their own sensor chips, buffers, reagents, 96 well plates, and other materials specific to their experiment. To schedule experiments, training, or services for the SPR, please contact Wendy Gavin at firstname.lastname@example.org prior to signing up on the CRF. Training takes from 2 to 4 hours depending on experience, qualitative analysis or quantitative analysis.