A Nanoemulsion of Antioxidant Synergy Formulation Enhanced Its Anti-inflammatory Properties In CD-1 Inflammatory Mice Model.
Robert Nicolosi, School of Health and Environment
Clinical and experimental evidences have suggested chronic inflammation may contribute to the development of diseases. Natural vitamin E consists of four different tocopherols that have varying degrees of antioxidant and anti-inflammatory properties. The aim of this study was to investigate whether the several nanoemulsion preparations of antioxidant synergy formulation (ASF) containing different tocopherol isomers would enhance the anti-inflammatory properties and bioavailability in CD-1 inflammatory mice models.
Inflammation was evaluated by changes of the earlobe thickness and induction of the cytokines expression. The results indicated that the earlobe thickness was significantly reduced by 50%, 50% and 73% in nanoemulsion of alpha, delta and gamma tocopherol, respectively. The cytokines TNF-α from earlobe homogenates was significantly reduced in nanoemulsion of alpha (-44%), delta (37%) and gamma (53%) tocopherol treated groups relative to the control group. Besides, IL-1α expression was also being reduced in delta (-31%) or gamma (-46%) encapsulated nanoemulsion treated groups. Bioavailability data for each tocopherols with the exception of alpha tocopherol suggest that the nanoemulsion preparations dramatically enhanced the bioavailability of gamma (220%) and delta (280%) tocopherol when compared to the equivalent dosage of the non-nanoemulsion preparations. These studies indicate that the nanoemulsion preparation of gamma, alpha, and delta tocopherols have enhanced anti-inflammatory properties in the CD-1 mouse that are associated with decreased earlobe thickness, the expression of TNF-α ,IL-1α and increased the bioavailability.