81.489 Practical Protein Crystallography

Practical Protein Crystallography

Course Details
Min Credits 4
Max Credits 4
Course ID 38015
Status Active

This course provides grounding in the principles and practice of protein x-ray crystallography. The course will be unique in format and provide both didactic and laboratory instruction. It is comprised of a series of lecture and laboratory exercises, with an emphasis on practical techniques and hands-on experience of modern protein crystallography. The course will cover the fundamental knowledge about x-ray physics, instrumentation and geometrical diffraction, protein crystallization, macromolecular data collection and processing, phase estimation and improvement, model building and refinement, and model assessment. Student will also be given a recently published structural paper for writhing a report on the subject.

Pre/Co-Requisites: Pre-req: 81.233 Experimental Methods in Biology, or 81.421 Biochemistry Techniques.