81.429 Recombinant Protein Production Techniques

Recombinant Protein Production Techniques

Course Details
Min Credits 4
Max Credits 4
Course ID 38228
Status Active

This course introduces students to the principles and practice of recombinant protein expression and purification's. Proteins are major targets of pharmaceuticals, and are themselves increasingly used as therapeuticals. However both basic research and pharmaceutical industry depends on availability of purified proteins that are often difficult to isolate from native sources. This course will provide both didactic and laboratory instruction. It is comprised of a series of lecture and laboratory exercises, with an emphasis on practical techniques and hands-on experience of recombinant protein purification. The course will cover a variety of expression systems, including prokaryotic and eukaryotic cells, and address traditional and new methods in protein purification.

Pre/Co-Requisites: Pre-req: 81.233 Experimental Methods in Biology, or 81.421 Biochemistry Techniques.