81.421 Biochemistry Techniques

Biochemistry Techniques

Course Details
Min Credits 2
Max Credits 2
Course ID 7515
Status Active

A series of discussions and "hands on" laboratory exercises emphasizing techniques and use of equipment most commonly employed in biochemicaI-biomedicaI research laboratories. Techniques to be mastered include: cell culture, cell fractionation, enzyme purification, ultracentrifugation, UV-visible spectrophotometry, spectrofluorometry, various types of chromatography (thin layer, gas, gel exclusion, ion exchange), electrophoresis, liquid scintillation spectrometry, and the safe handling and application of radioisotopes to problems in biochemistry. Wherever possible, the principles presented in 81.419 will be used as a basis for experimentation using the above techniques.

Pre/Co-Requisites: Pre-req: 81.233 Experimental Methods in Biology, and 84.230 Organic Chem Lab II.