36.580 Clinical Applications of Molecular Genetics

Clinical Applications of Molecular Genetics

Course Details
Min Credits 3
Max Credits 3
Course ID 4848
Status Active

This course begins with a review of basic molecular genetics, followed by a discussion of the human genome project, including the application of its technology to the management of genetic diseases. Laboratory techniques used to analyze pre- and postnatal tissue samples is described, including RFLP-Southern blot technology, real- time PCR, VNTR assessment, and gene expression analysis by microchip arrays. Cloning and stem cell analysis follows, including the ethical, legal, and social issues surrounding these areas. Cancer genetics and cytogenesis is presented, with emphasis on molecular changes that lead to the onset of cancer. Genetic treatments for cancer are reviewed. Gene therapy is detailed, including the historical perspective, gene therapy vectors and their mode of action, and the newest methods for treating genetic disorders at the DNA level. Structural and functional genomics follows, centering around the creation of pharmaceuticals solely based on the genetic basis of diseases. The course ends with a discussion of the human proteome project, including goals and objectives, current progress, and the storage of data in complex bioinformatic databases.